Document 0084
 DOCN  M9460084
 TI    Specific diagnosis of progressive multifocal leukoencephalopathy by
       polymerase chain reaction.
 DT    9408
 AU    Weber T; Turner RW; Frye S; Ruf B; Haas J; Schielke E; Pohle HD; Luke W;
       Luer W; Felgenhauer K; et al; Neurologische Klinik und Poliklinik,
       Georg-August-Universitat,; Gottingen, Germany.
 SO    J Infect Dis. 1994 May;169(5):1138-41. Unique Identifier : AIDSLINE
       MED/94223080
 AB    Using polymerase chain reaction (PCR), 34 cerebrospinal fluid (CSF)
       samples from 28 patients with progressive multifocal leukoencephalopathy
       (PML) were analyzed. As controls, 116 samples were evaluated from 82
       human immunodeficiency virus type 1 (HIV-1)-infected patients and 1
       HIV-1-negative patient. Of the HIV-1-positive patients, 23 had cerebral
       toxoplasmosis, 10 had HIV leukoencephalopathy, and 49 had other
       neurologic complications. Detection of JC virus (JCV) DNA in CSF was
       increased 10-fold by the addition of carrier DNA before
       phenol-chloroform-isoamyl alcohol extraction. The primer pair JC 26/29,
       from the VP1/large T region, had a limit of detection of 10(5) JCV DNA
       molecules/100 microL. The primer pair JC 36/39, located in the large T
       gene region, had a 100-fold lower limit of detection. With JC 26/29, the
       sensitivity was 43% (12/28) and specificity was 100%. Using JC 36/39,
       sensitivity increased to 82% (23/28), and false-positive results were
       not observed. Diagnosis of PML is greatly aided by PCR analysis of CSF.
 DE    Base Sequence  DNA, Viral  Human  HIV Infections/COMPLICATIONS
       Leukoencephalopathy, Progressive Multifocal/CEREBROSPINAL FLUID/
       COMPLICATIONS/*DIAGNOSIS  Molecular Sequence Data  *Polymerase Chain
       Reaction  Polyomavirus/GENETICS/*ISOLATION & PURIF  Sensitivity and
       Specificity  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).