Document 0115
 DOCN  M9460115
 TI    Effect of serine protease inhibitor, N-alpha-tosyl-L-lysyl-chloromethyl
       ketone (TLCK), on cell-mediated and cell-free HIV-1 spread.
 DT    9408
 AU    Bourinbaiar AS; Nagorny R; Department of Biochemistry, New York
       University Medical Center,; New York 10016.
 SO    Cell Immunol. 1994 Apr 15;155(1):230-6. Unique Identifier : AIDSLINE
       MED/94221660
 AB    The effect of a serine protease inhibitor,
       N-alpha-tosyl-L-lysyl-chloromethyl ketone (TLCK), on cell adhesion and
       resulting cell-to-cell HIV-1 transmission was examined. Cell-mediated
       viral spread was blocked by 10(-4) M TLCK--the nontoxic dose at which
       the maximum inhibition of cell adhesion was achieved. TLCK
       (10(-4)-10(-8) M range) exhibited a similar dose-dependent effect when
       tested against cell-free virus infection. These findings suggest that
       TLCK acts by preventing both cell-cell adhesion and viral binding to the
       target cell. The effect of TLCK could be attributed to an irreversible
       modification of surface and/or intracellular proteases required for
       functional activation of HIV-1 envelope glycoproteins.
 DE    Cell Adhesion/*DRUG EFFECTS  Dose-Response Relationship, Drug  Human
       HIV-1/*GROWTH & DEVELOPMENT  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*MICROBIOLOGY  Tosyllysine Chloromethyl
       Ketone/*PHARMACOLOGY  Virus Replication/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).