Document 0188
 DOCN  M9460188
 TI    Multiple effects of CD4 CDR3-related peptide derivatives showing
       anti-HIV-1 activity on HIV-1 gp120 functions.
 DT    9408
 AU    Ohki K; Kimura T; Jones IM; Morita F; Ikuta K; Section of Serology,
       Hokkaido University, Sapporo, Japan.
 SO    Vaccine. 1994 Mar;12(4):343-50. Unique Identifier : AIDSLINE
       MED/94233850
 AB    The interaction of the human immunodeficiency virus type 1 (HIV-1)
       envelope glycoprotein gp120 with CD4 CDR3-related peptide derivatives
       showing anti-HIV-1 activity has been studied. Conformational changes in
       gp120, which could affect its interaction with CD4 and its shedding from
       virions, were detected by fluorescence spectrum analysis of tryptophan
       residues after addition of peptide representative of the CD4
       CDR3-related region, but not the CD4 CDR2-related region. Interestingly,
       the addition of scrambled peptide, S1 (with altered amino acid sequence
       compared with the native CDR3-related peptide but unaltered overall
       composition), which we recently showed to have stronger anti-HIV-1
       activity than the original CDR3-related peptide, had no effects on the
       conformational change in gp120 or on its interaction with CD4 and its
       shedding from HIV-1 virions. However, all of the CDR3-related peptides,
       including S1, showed blocking effects on the binding of antibodies
       against gp120 V3 loop and C-terminus regions. Thus, we concluded that
       there were at least two separable activities of the CDR3-related
       peptides in anti-HIV-1 activity, i.e. induction of conformational
       changes in gp120, which could affect its binding to CD4 and to gp41 (as
       observed in native CDR3-related peptides), and inactivation of V3 loop
       and C-terminus regions in gp120 (as observed in all of the CDR3-related
       peptides, including S1).
 DE    Amino Acid Sequence  Antibodies, Monoclonal/IMMUNOLOGY  Antigens,
       CD4/*IMMUNOLOGY  Cell Line  Enzyme-Linked Immunosorbent Assay  Flow
       Cytometry  Human  HIV Antibodies/IMMUNOLOGY  HIV Envelope Protein
       gp120/*IMMUNOLOGY/METABOLISM  HIV-1/*IMMUNOLOGY  Immunoglobulin Variable
       Region/IMMUNOLOGY  Molecular Sequence Data  Peptide
       Fragments/*IMMUNOLOGY  Protein Conformation  Support, Non-U.S. Gov't
       Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).