Document 0195
 DOCN  M9460195
 TI    The difference in gp160 and gp120 of HIV type 1 in the induction of CD4
       downregulation preceding single-cell killing.
 DT    9408
 AU    Koga Y; Nakamura K; Sasaki M; Kimura G; Nomoto K; Department of
       Infectious Disease, School of Medicine, Tohkai; University, Kanagawa,
       Japan.
 SO    Virology. 1994 May 15;201(1):137-41. Unique Identifier : AIDSLINE
       MED/94233762
 AB    The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein is
       synthesized as a precursor glycoprotein, gp160, and is then processed
       into gp120 and gp41. In the present study, CD4+ cell clones expressing
       either gp160 or gp120 of HIV-1 under the transcriptional control of an
       inducible promoter were made in order to examine the effect of these env
       products on the downregulation of surface CD4 and cell injury. A
       complete disappearance of surface CD4 preceding single-cell death
       occurred in the cell clones expressing gp160, in which a complex between
       CD4 and gp160 was formed and then accumulated intracellularly. In
       contrast to this, the cell clones expressing gp120 neither exhibited any
       such depletion of surface CD4 nor showed any apparent cytopathic effect.
       Therefore, it is thought that gp160 but not gp120 plays a crucial role
       in both the downregulation of surface CD4 and the resultant cell death
       in the cells infected with HIV-1.
 DE    Antigens, CD4/*METABOLISM  Base Sequence  *Cell Death  Cell Line
       Cytopathogenic Effect, Viral  Down-Regulation (Physiology)  Gene
       Products, env/GENETICS/*PHYSIOLOGY  Genes, env/GENETICS  Human  HIV
       Envelope Protein gp120/GENETICS/*PHYSIOLOGY  HIV-1/*PATHOGENICITY
       Molecular Sequence Data  Monocytes/CYTOLOGY/MICROBIOLOGY  Mutagenesis,
       Site-Directed  Protein Precursors/GENETICS/*PHYSIOLOGY  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).