Document 0199
 DOCN  M9460199
 TI    HIV-1 gene expression and replication in neuronal and glial cell lines
       with immature phenotype: effects of nerve growth factor.
 DT    9408
 AU    Ensoli F; Ensoli B; Thiele CJ; Cell and Molecular Biology Section,
       National Cancer Institute,; National Institutes of Health, Bethesda,
       Maryland 20892.
 SO    Virology. 1994 May 1;200(2):668-76. Unique Identifier : AIDSLINE
       MED/94233730
 AB    Encephalopathy and neurological disorders are a major manifestation of
       pediatric AIDS. Although HIV-1 can replicate in cells of neuronal and
       glial origin, it is yet unclear whether immature neural cells, which are
       present during nervous system development, can support HIV-1 replication
       and whether neurotrophic factors can modulate HIV-1 gene expression. In
       this study we show that a glial cell line with a phenotype closely
       resembling immature glial cells is more permissive to HIV-1 infection
       and replication than a neuroblastic cell line. After HIV-1 infection or
       after transfection of these cells with the HIV-1 LTR-CAT reporter gene
       alone or in the presence of Tat, both HIV-1 replication and viral gene
       expression progressively decrease in the neuronal cell line, while they
       increase in the glial cell line. In both cell types viral gene
       expression and replication are augmented by the addition to the cells of
       nerve growth factor (NGF) at concentrations which induce neuronal
       differentiation. However, these effects are again more evident with the
       glial cell type, suggesting that immature glial cells may represent one
       of the major targets and reservoirs of HIV-1 in the developing nervous
       system. As NGF and Tat act synergistically in inducing HIV-1 gene
       expression, these data also suggest that during development the presence
       of high levels of neural trophic factors may activate viral replication
       and render the CNS more susceptible to the deleterious effects of HIV-1
       infection.
 DE    Cell Differentiation/DRUG EFFECTS  Cell Line  Comparative Study  Gene
       Expression/DRUG EFFECTS  Gene Products, tat/PHARMACOLOGY  Genes,
       Reporter  Human  HIV Long Terminal Repeat/GENETICS  HIV-1/*GROWTH &
       DEVELOPMENT  Nerve Growth Factors/*PHARMACOLOGY  Neuroglia/*MICROBIOLOGY
       Neurons/*MICROBIOLOGY  Phenotype  Stem Cells/MICROBIOLOGY
       Trans-Activation (Genetics)  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).