Document 0264
 DOCN  M9460264
 TI    Diagnosis and differentiation of HIV-1 and HIV-2 infection by two rapid
       assays in Nigeria.
 DT    9408
 AU    Kline RL; Dada A; Blattner W; Quinn TC; Laboratory of Immunoregulation,
       National Institute of Allergy and; Infectious Diseases, National
       Institutes of Health, Bethesda,; Maryland.
 SO    J Acquir Immune Defic Syndr. 1994 Jun;7(6):623-6. Unique Identifier :
       AIDSLINE MED/94231468
 AB    We evaluated the reliability, sensitivity, and specificity of two rapid
       assays, TestPack HIV-1/HIV-2 and Genie HIV-1/HIV-2, in Lagos, Nigeria.
       An alternative algorithm to EIA and Western blot was then examined with
       the TestPack HIV-1/HIV-2 used as the screening test and the Genie
       HIV-1/HIV-2 used as the supplemental test to differentiate HIV-1 and
       HIV-2 infection. In all, 845 prostitutes were evaluated for HIV-1 and
       HIV-2 infection using one of the two rapid tests and compared to EIA and
       Western blot results. Of these 845 cases, 437 samples were analyzed by
       both assays. Overall, 109 (12.7%) prostitutes were antibody positive for
       HIV-1, 13 (1.5%) for HIV-2, and six (0.7%) were dually reactive for both
       HIV-1 and HIV-2. Compared to Western blot, the Genie HIV-1/HIV-2 had a
       slightly higher sensitivity and specificity (98.4% and 99.7%) than the
       TestPack HIV-1/HIV-2 (97.6% and 99.3%). The alternative algorithm using
       both rapid assays had a sensitivity of 96.9% and a specificity of 99.9%.
       The Genie HIV-1/HIV-2 correctly identified 104 of 108 HIV-1 positive
       sera, 12 of 13 HIV-2 positive sera, and all six dually positive sera.
       Both assays performed well in the field. They required < 10 min to
       complete, no equipment, and little training. An algorithm incorporating
       two rapid assays can be used as a less expensive alternative to
       traditional testing strategies comparable in reliability to ELISA and
       Western blot; it provides the additional advantage of differentiating
       between HIV-1 and HIV-2.
 DE    Algorithms  Blotting, Western  Comparative Study  Diagnosis,
       Differential  Evaluation Studies  Human  HIV Antibodies/*BLOOD  HIV
       Infections/*DIAGNOSIS  HIV-1/*IMMUNOLOGY  HIV-2/*IMMUNOLOGY
       Immunoenzyme Techniques  Nigeria  Prostitution  Reproducibility of
       Results  Sensitivity and Specificity  JOURNAL ARTICLE

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