Document 0116
 DOCN  M9470116
 TI    HTLV-I messenger RNA is expressed in vivo in adult T-cell
       leukemia/lymphoma patients: an in situ hybridization study.
 DT    9409
 AU    Setoyama M; Fujiyoshi T; Mizoguchi S; Katahira Y; Yashiki S; Tara M;
       Kanzaki T; Sonoda S; Department of Dermatology, Faculty of Medicine,
       Kagoshima; University, Japan.
 SO    Int J Cancer. 1994 Jun 1;57(5):760-4. Unique Identifier : AIDSLINE
       MED/94252726
 AB    Adult T-cell leukemia/lymphoma (ATLL) is a malignancy involving
       peripheral blood, lymph nodes, skin and other organs. Human T-cell
       leukemia virus type I (HTLV-I) is etiologically associated with ATLL but
       cannot be detected by conventional methods in fresh samples of
       peripheral blood and skin taken from ATLL. The aim of this study was to
       investigate the feasibility of an in situ hybridization technique for
       detection of HTLV-I mRNA in atypical lymphoid cells of peripheral blood
       and skin lesions of patients with ATLL. We detected variable amounts of
       HTLV-I tax mRNA in the nuclei and cytoplasm of these cells in fresh
       peripheral blood samples and skin lesions from ATLL patients, and also
       in asymptomatic HTLV-I infected donors to a lesser extent. Out of 10
       patients with ATLL, 7 showed strong positive in situ hybridization
       whereas the other 3 were only weakly positive. However, in the last 3
       cases, the reaction became strongly positive after cells had been
       cultured for 24 hr. Furthermore, all 3 asymptomatic HTLV-I-infected
       donors exhibited a weakly positive response in their apparently mature
       lymphoid cells.
 DE    Adult  Aged  Female  Gene Expression Regulation, Viral  Genes, pX  Human
       HTLV-I/*GENETICS  In Situ Hybridization  Leukemia-Lymphoma, T-Cell,
       Acute, HTLV-I-Associated/*GENETICS  Lymphocytes/MICROBIOLOGY  Male
       Middle Age  Oligonucleotide Probes  RNA, Messenger/GENETICS  RNA,
       Viral/GENETICS  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).