Document 0156
 DOCN  M9470156
 TI    Trichosanthin, a ribosome inactivating and anti-HIV protein, does not
       alter the concentration of beta-endorphin in the mouse brain.
 DT    9409
 AU    Ng TB; Kwong WH; Yeung HW; Department of Biochemistry, Faculty of
       Medicine, Chinese; University of Hong Kong.
 SO    Biochem Mol Biol Int. 1993 Dec;31(6):1101-4. Unique Identifier :
       AIDSLINE MED/94251174
 AB    Trichosanthin was administered intramuscularly into mature male ICR mice
       at a dosage of 0.2mg/25g, daily for three days. The mice were sacrificed
       four hours after the last injection. Their brains were dissected out and
       divided into three regions: A(thalamus and hypothalamus), B(cerebral
       cortex) and C(cerebellum and brainstem). Their pituitaries were also
       removed. The pituitaries and the various brain regions were extracted
       with hot 1M acetic acid. The extracts were lyophilized and reconstituted
       in radioimmunoassay (RIA) buffer before assay for beta-endorphin by RIA.
       It was found that there was a significant elevation of beta-endorphin
       concentration after trichosanthin treatment only in brain region A. The
       data suggest that trichosanthin does not adversely affect the brain.
 DE    beta-Endorphin/*METABOLISM  Animal  Antiviral Agents/PHARMACOLOGY  Brain
       Chemistry/*DRUG EFFECTS  Hypothalamus/DRUG EFFECTS/METABOLISM  HIV/DRUG
       EFFECTS  Male  Mice  Mice, Inbred ICR  Pituitary Gland/DRUG
       EFFECTS/METABOLISM  Radioimmunoassay  Ribosomes/DRUG EFFECTS
       Thalamus/DRUG EFFECTS/METABOLISM  Trichosanthin/*PHARMACOLOGY  JOURNAL
       ARTICLE

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