Document 0776
 DOCN  M9470776
 TI    [Primers for the nef gene in the diagnosis of HIV infection using the
       polymerase chain reaction]
 DT    9409
 AU    Radiuk SN; Matsevich GR; Tarantul VZ; Andzhaparidze OG
 SO    Vopr Virusol. 1994 Mar-Apr;39(2):53-6. Unique Identifier : AIDSLINE
       MED/94287653
 AB    A new system of primers and internal probe identifying the nef gene of
       HIV-1 was selected and tested for the laboratory diagnosis of HIV
       infection by means of PCR. The proposed primers and probe by their
       characteristics approached the optimal ones for PCR diagnosis. The
       diagnostic value of the above system was confirmed by the PCR analysis
       of blood samples from 29 patients including subjects with a doubtful
       diagnosis of HIV infection. The results of the PCR analysis using
       primers nef1/nef2 correlated with the results obtained by employing the
       widely used primers for the gag gene SK38/SK39 and attested to the high
       specificity and diagnostic efficiency of the proposed system.
 DE    Acquired Immunodeficiency Syndrome/DIAGNOSIS/GENETICS  Adolescence
       Adult  Child  Child, Preschool  Comparative Study  DNA
       Primers/*DIAGNOSTIC USE/GENETICS  English Abstract  Genes, nef/*GENETICS
       Human  HIV Antibodies/BLOOD  HIV Infections/*DIAGNOSIS/GENETICS  HIV
       Seronegativity/GENETICS/IMMUNOLOGY  HIV-1/*GENETICS/IMMUNOLOGY  Middle
       Age  Polymerase Chain Reaction/*METHODS  Sensitivity and Specificity
       JOURNAL ARTICLE

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