Document 0860
 DOCN  M9470860
 TI    Epstein-Barr-encoded RNA in situ hybridization: diagnostic applications.
 DT    9409
 AU    Ambinder RF; Mann RB; Department of Oncology, Johns Hopkins School of
       Medicine,; Baltimore, MD.
 SO    Hum Pathol. 1994 Jun;25(6):602-5. Unique Identifier : AIDSLINE
       MED/94283949
 AB    Latent Epstein-Barr virus (EBV) infection is associated with a variety
       of malignancies and other diseases. Highly restricted viral antigen
       expression and low viral genome copy number in infected tissues impede
       conventional immunohistochemical or in situ hybridization approaches to
       the detection of virus in these tissues. In situ hybridization detection
       of two small but very abundant nuclear RNAs known as the EBERs serves as
       an alternative approach. The EBERs are stable over time and can be
       detected in all common fixatives. This technique facilitates
       characterization of the cellular locus of latent EBV infection in
       histologically complex tissues, such as Hodgkin's disease and
       angioimmunoblastic lymphadenopathy with dysproteinemia.
 DE    Herpesviridae Infections/*DIAGNOSIS  Herpesvirus 4, Human/*GENETICS
       Hodgkin's Disease/MICROBIOLOGY  Human  In Situ Hybridization  RNA,
       Viral/*CHEMISTRY  Support, Non-U.S. Gov't  Tumor Virus
       Infections/*DIAGNOSIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).