Document 0861
 DOCN  M9470861
 TI    Antigenic characterization of human isolates of cryptosporidia.
 DT    9409
 AU    Ditrich O; Kopacek P; Kucerova Z; Institute of Parasitology, Academy of
       Sciences of the Czech; Republic, Ceske Budejovice.
 SO    Folia Parasitol (Praha). 1993;40(4):301-5. Unique Identifier : AIDSLINE
       MED/94283926
 AB    Isolates of cryptosporidia from immunodeficient patients and from
       immunocompetent children suffering from diarrhoea were sources for
       preparation of antigens. Altogether, antigens from 21 isolates were
       obtained, 19 from children and 2 from AIDS patients. With one exception,
       all children were under 4 years old and most of them were between 1 and
       2 years old. The probable source of infection was found in 11 cases. In
       8 cases, as well as in both AIDS patients, the source of infection was
       not determined. The same groups of persons and the control group of
       healthy blood donors were examined serologically using ELISA with the
       antigen of bovine origin and positive sera were used for following
       experiments. Soluble and membrane-enriched antigens from oocysts were
       compared using polyacrylamide gel electrophoresis and
       electroimmunotransfer blots developed by human immune sera. While no
       differences were recorded in soluble antigens, two of the
       membrane-enriched antigens were missing (15.5 and 33 kD bands). While
       all isolates with a pattern typical for Cryptosporidium parvum were
       infective for neonatal mice, both isolates with missing bands were not
       infective for newborn mice in repeated experiments. The first of them
       was infective for chickens and was originally determined to be
       Cryptosporidium baileyi (Ditrich et al. 1991). However, the antigenic
       pattern differs from pattern of this species. The second isolate was
       infective for guinea pigs and its species classification remains
       uncertain.
 DE    Adolescence  Animal  Antibodies, Protozoan/BLOOD  Antigens,
       Protozoan/*ISOLATION & PURIF  Child  Child, Preschool  Comparative Study
       Cryptosporidiosis/*COMPLICATIONS
       Cryptosporidium/CLASSIFICATION/*IMMUNOLOGY  Diarrhea/PARASITOLOGY  Human
       HIV Seropositivity/COMPLICATIONS/PARASITOLOGY  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).