Document 0865
 DOCN  M9470865
 TI    Staining of microsporidian spores by optical brighteners with remarks on
       the use of brighteners for the diagnosis of AIDS associated human
       microsporidioses.
 DT    9409
 AU    Vavra J; Dahbiova R; Hollister WS; Canning EU; Department of
       Parasitology, Charles University, Prague, Czech; Republic.
 SO    Folia Parasitol (Praha). 1993;40(4):267-72. Unique Identifier : AIDSLINE
       MED/94283919
 AB    Conditions for the effective fluorescence labelling of microsporidian
       spores by optical brighteners, based on the presence of chitin in the
       spore wall, are described. Spores of Vairimorpha ephestiae, V. necatrix,
       V. plodiae, Nosema bombycis, N. apis, N. algerae, Encephalitozoon
       cuniculi and Enterocytozoon bieneusi were examined. The degree of
       binding of Calcofluor White M2R (CFW) to untreated spores depends on the
       conditions and time of storage and the degree of bacterial contamination
       of the spore sample. Unpurified spores, stored in water are unreliable
       as control material for the estimation of CFW labelling. However, spores
       subjected to alkaline treatment by NaOH before CFW application are
       visualized with ease under all experimental conditions by their bright,
       not quenching fluorescence in shortwave light (approximately 350 nm) in
       CFW dilutions of 10(-4) or even lower. Similar improvement in labelling
       is achieved by exposing spores to CFW dissolved in 0.5-1N NaOH. As well
       as Calcofluor White M2R other optical brighteners (e.g. Uvitex 2B,
       Ciba-Geigy or Rylux BA, Ostacolor) can be used for labelling of spores.
 DE    Animal  AIDS-Related Opportunistic Infections/*PARASITOLOGY  Human
       Microscopy, Fluorescence  Microspora Infections/*DIAGNOSIS
       Microsporida/*CYTOLOGY  Spores/CYTOLOGY  Stains and Staining/*METHODS
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).