Document 0866
 DOCN  M9470866
 TI    The Warthin-Starry stain in the diagnosis of small intestinal
       microsporidiosis in HIV-infected patients.
 DT    9409
 AU    Field AS; Marriott DJ; Hing MC; Department of Anatomical Pathology, St.
       Vincent's Hospital,; Darlinghurst, Sydney, Australia.
 SO    Folia Parasitol (Praha). 1993;40(4):261-6. Unique Identifier : AIDSLINE
       MED/94283918
 AB    A protocol for the handling of small intestinal biopsies from
       HIV-infected patients is presented. This protocol includes the
       Warthin-Starry stain for the detection of microsporidia. This stain has
       proved a reliable and sensitive diagnostic technique for microsporidial
       infections as it stains both Enterocytozoon bieneusi and Septata
       intestinalis in duodenal enterocytes. Because the stain demonstrates
       Septata intestinalis in lamina propria macrophages as well as
       enterocytes, it allows for the practical differentiation of these two
       microsporidial infections. The Warthin-Starry stain has also
       demonstrated Septata intestinalis in nasal and colonic biopsies in some
       of these patients. Since the completion of an earlier study, a further
       40 cases of Enterocytozoon bieneusi and three cases of Septata
       intestinalis have been diagnosed in just over 240 consecutive duodenal
       biopsies from HIV positive patients presenting with diarrhoea and other
       gastrointestinal complaints. Other opportunistic infections include
       cytomegalovirus in four cases, mycobacteria in eight cases,
       cryptosporidia in nine cases, giardia in four cases and Isospora belli
       in one case. Since the ratio of these opportunistic infections has
       remained much the same as in the previous study of 180 consecutive
       duodenal biopsies, we suggest that these rates may reflect the actual
       prevalence of microsporidial infections in AIDS patients in Sydney,
       Australia.
 DE    Animal  AIDS-Related Opportunistic Infections/*PARASITOLOGY  Biopsy
       Duodenum/CYTOLOGY/*PARASITOLOGY  Encephalitozoon/CYTOLOGY  Human
       Microspora Infections/*DIAGNOSIS  Stains and Staining/*METHODS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).