Document 0895
 DOCN  M9470895
 TI    ELISA-type titertray assay of IgM anti-GM1 autoantibodies.
 DT    9409
 AU    Bech E; Jakobsen J; Orntoft TF; Department of Clinical Chemistry, Aarhus
       University Hospital,; Denmark.
 SO    Clin Chem. 1994 Jul;40(7 Pt 1):1331-4. Unique Identifier : AIDSLINE
       MED/94282916
 AB    We report an ELISA-type titertray assay for autoantibodies against the
       ganglioside GM1. Trays were coated with ganglioside GM1 and reacted with
       patients' sera; bound IgM was detected with rabbit antibody to human
       IgM. High-titer serum from a patient was used as calibrator, another
       patient's serum as the positive control, and the GM1-specific cholera
       toxin as the control for GM1 coating. Regression curves of serum titers
       obtained from different patients were linear and parallel. Intra- and
       interassay CVs were 4.0-7.8% and 5.5-16%, respectively. We detected
       antibodies at a titer of 1:250 in normal subjects. Analytical
       specificity of the calibrator serum against GM1 was demonstrated by
       immune thin-layer chromatography. Anti-GM1 antibodies were increased in
       patients with chronic inflammatory demyelinating polyradiculoneuropathy
       (P < 0.002) or multiple sclerosis (P < 0.01). In Guillain-Barre
       syndrome, preliminary longitudinal studies showed a decrease in anti-GM1
       titer that was related to clinical recovery.
 DE    Alcohol, Ethyl  Autoantibodies/*BLOOD  Carcinoma, Small Cell/IMMUNOLOGY
       Drug Stability  Enzyme-Linked Immunosorbent Assay/*METHODS/STATISTICS &
       NUMER  DATA  G(M1) Ganglioside/*IMMUNOLOGY  Human  HIV
       Seropositivity/IMMUNOLOGY  IgM/*BLOOD  Lung Neoplasms/IMMUNOLOGY  Motor
       Neuron Disease/IMMUNOLOGY  Multiple Sclerosis/IMMUNOLOGY
       Polyradiculoneuritis/IMMUNOLOGY  Sensitivity and Specificity  Solvents
       JOURNAL ARTICLE

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