Document 0914
 DOCN  M9470914
 TI    Disseminated Mycobacterium genavense infection in two patients with
       AIDS.
 DT    9409
 AU    Gaynor CD; Clark RA; Koontz FP; Emler S; Hirschel B; Schlesinger LS;
       Department of Medicine, University of Iowa Hospitals and Clinics,; Iowa
       City 52242.
 SO    Clin Infect Dis. 1994 Mar;18(3):455-7. Unique Identifier : AIDSLINE
       MED/94281435
 AB    Mycobacterium genavense is a recently defined fastidious organism that
       has been identified as a cause of disseminated infection in patients
       with AIDS. We report the cases of two patients who had advanced AIDS and
       a clinical syndrome of fever, anorexia, abdominal pain, diarrhea, and
       weight loss. In addition, splenomegaly and lymphadenopathy were
       prominent in both cases, and in one patient's case radiographic findings
       were suggestive of splenic abscesses. Mycobacteria isolated from
       specimens of blood and bone marrow grew in liquid media but not on solid
       media. The results of DNA probe tests for Mycobacterium tuberculosis and
       Mycobacterium avium complex were false-positive for both patients. After
       treatment of the broth cultures to lyse red blood cells, the results of
       DNA probe tests were negative for these pathogens. Amplification and
       sequencing of 16S rRNA with use of the polymerase chain reaction
       indicated that the mycobacterial isolates from both patients had
       sequences identical to those previously reported for M. genavense. One
       patient survived 5 months after diagnosis, the other 2 months after
       diagnosis; only one patient responded (transiently) to antimycobacterial
       chemotherapy.
 DE    Adult  AIDS-Related Opportunistic Infections/*COMPLICATIONS/DIAGNOSIS/
       MICROBIOLOGY  Base Sequence  Case Report  Comparative Study  DNA Probes
       Human  Male  Molecular Sequence Data  Mycobacterium/GENETICS/ISOLATION &
       PURIF  Mycobacterium avium Complex/GENETICS  Mycobacterium
       tuberculosis/GENETICS  Mycobacterium
       Infections/*COMPLICATIONS/DIAGNOSIS/MICROBIOLOGY  RNA,
       Bacterial/GENETICS  RNA, Ribosomal, 16S/GENETICS  Species Specificity
       JOURNAL ARTICLE  REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).