Document 0970
 DOCN  M9470970
 TI    Lack of evidence for a superantigen in lymphocytes from HIV-discordant
       monozygotic twins.
 DT    9409
 AU    Nisini R; Aiuti A; Matricardi PM; Fattorossi A; Ferlini C; Biselli R;
       Mezzaroma I; Pinter E; D'Amelio R; Dipartimento di Biopatologia Umana,
       Universita La Sapienza di; Roma, Italy.
 SO    AIDS. 1994 Apr;8(4):443-9. Unique Identifier : AIDSLINE MED/94280715
 AB    OBJECTIVE: An HIV-associated superantigen (SAg) has been hypothesized.
       Here we test whether an SAg is functionally detectable in peripheral
       blood mononuclear cells (PBMC) from monozygotic twins discordant for HIV
       infection. DESIGN AND METHODS: The V beta selective T-cell depletion
       found in minor lymphocyte stimulation (Mls)-positive mice is caused by
       an SAg encoded by the mouse mammary tumour virus. Mls is a locus whose
       gene product stimulates a mixed lymphocyte reaction (MLR) in mice
       strains identical at the major histocompatibility complex locus. If an
       SAg is present in PBMC and/or sorted CD4+ cells from one HIV-infected
       monozygotic twin, it would stimulate PBMC from the corresponding healthy
       monozygotic twin in an MLR. In addition, if an SAg causes V
       beta-selective T-cell depletion in AIDS patients, a differential
       proliferation to a panel of staphylococcal enterotoxins (SE) of T
       lymphocytes from healthy and HIV-infected monozygotic twins should
       become measurable. RESULTS: No positive MLR or significant differences
       in the SE-driven proliferation between the healthy and the HIV-infected
       twins were observed. CONCLUSIONS: Our results suggest that PBMC from the
       two HIV-infected twins do not express a functionally detectable SAg.
 DE    Adult  Animal  Cells, Cultured  *Diseases in Twins  Female  Human
       HIV/*IMMUNOLOGY  HIV Infections/*IMMUNOLOGY  Lymphocyte Culture Test,
       Mixed  Lymphocytes/CYTOLOGY/*IMMUNOLOGY  Male  Mice  Middle Age
       Superantigens/*IMMUNOLOGY  Support, Non-U.S. Gov't  *Twins, Monozygotic
       JOURNAL ARTICLE

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