Document 0973
 DOCN  M9470973
 TI    Peptide serology for analysis of the inter- and intra-individual
       variation in the HIV-1 V3 domain.
 DT    9409
 AU    Jansson M; Wahren B; Albert J; Scarlatti G; Franzen L; Kataaha PK;
       Katabira E; Mubiro F; Wigzell H; Rossi P; Department of Immunology,
       Karolinska Institute, Stockholm,; Sweden.
 SO    AIDS. 1994 Apr;8(4):413-21. Unique Identifier : AIDSLINE MED/94280712
 AB    OBJECTIVE: To investigate whether the specificity of antibody responses
       to the gp120 V3 domain in HIV-1-infected individuals is related to the
       variability of this region. METHODS: Sera from a cohort of 22
       HIV-1-infected Ugandans were tested against peptides derived from each
       individual's autologous proviral V3 apex sequence. Autologous peptide
       reactivity was compared with reactivity to peptides derived from two
       Ugandan consensus sequences and previously isolated US/European and
       African viruses. Peptides from individuals with heterogeneous V3 apex
       sequences, representing different HIV-1 variants, were obtained and
       tested against the corresponding sera. RESULTS: A notable
       cross-reactivity to different V3 apex peptides was observed. However, in
       the majority of sera, antibody reactivity to the autologous peptides was
       found to exceed reactivity to any of the other peptides tested. V3
       proviral sequences from the Ugandan cohort studied have been shown to be
       closely related to the HIV-1MN isolate and thus, their sera gave better
       reactivity to V3MN and related peptides than to peptides representing
       other African HIV-1 isolates. In individuals with heterogeneous V3
       proviral sequences, we could distinguish divergent antibody responses to
       the genomic variants differing by single amino acids. CONCLUSION:
       Analysis of seroreactivity to peptides might constitute a relevant tool
       for investigating the variability of the HIV-1 gp120 V3 domain within
       infected populations and single individuals.
 DE    Amino Acid Sequence  Antibody Specificity  *Antigenic Variation  Cross
       Reactions  Enzyme-Linked Immunosorbent Assay  Human  HIV
       Antibodies/IMMUNOLOGY  HIV Envelope Protein gp120/*IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Molecular Sequence Data
       Peptide Fragments/*IMMUNOLOGY  Support, Non-U.S. Gov't  Uganda  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).