Document 0981
 DOCN  M9470981
 TI    Progressive multifocal leukoencephalopathy diagnosed by amplification of
       JC virus-specific DNA from cerebrospinal fluid.
 DT    9409
 AU    Weber T; Turner RW; Frye S; Luke W; Kretzschmar HA; Luer W; Hunsmann G;
       Neurologische Klinik und Poliklinik, Georg-August-Universitat;
       Gottingen, Germany.
 SO    AIDS. 1994 Jan;8(1):49-57. Unique Identifier : AIDSLINE MED/94280704
 AB    OBJECTIVE: To study the diagnostic sensitivity and specificity of
       polymerase chain reaction (PCR) for the non-invasive diagnosis of
       progressive multifocal leukoencephalopathy (PML) in HIV-1-infected
       individuals. DESIGN: Retrospective analysis of stored cerebrospinal
       fluid (CSF) samples by PCR of HIV-1-infected patients. METHODS: Results
       of the PCR analysis of the CSF of three AIDS patients with
       autopsy-proven PML were compared with the results in 15 neurologically
       asymptomatic HIV-1-infected patients and with 15 AIDS patients with
       other opportunistic infections of the central nervous system (CNS). A
       polyclonal antiserum to simian virus 40 (SV40) cross-reacting with JC
       virus (JCV) late antigens was used for immunocytochemical confirmation
       of the diagnosis. Two different primer pairs, one taken from the
       VP1/large T gene and the other from the large T gene, were used to
       amplify JCV-specific DNA sequences from CSF. RESULTS: Five CSF samples
       were analysed and JCV-specific DNA found in three patients with
       autopsy-proven PML. No JCV-specific DNA was detected in 47 CSF samples,
       including serial samples from 14 of the 30 non-PML patients. The
       diagnosis of PML was confirmed in all three cases by
       immunocytochemistry. CONCLUSION: PML can be diagnosed by PCR analysis of
       CSF. The sensitivity and specificity of the method depends on the
       sensitivity of the primers used for amplification. Using a primer pair
       from the large T gene, JCV-specific DNA was amplified in three cases
       with PML as early as the day of presentation with the first neurological
       symptom of PML.
 DE    Adult  AIDS-Related Opportunistic Infections/*DIAGNOSIS  Base Sequence
       Case Report  DNA, Viral/*CEREBROSPINAL FLUID  Female  Human  *HIV-1
       Leukoencephalopathy, Progressive Multifocal/*DIAGNOSIS  Male  Molecular
       Sequence Data  Polymerase Chain Reaction/*METHODS  Polyomavirus hominis
       2/*GENETICS  Retrospective Studies  Sensitivity and Specificity
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).