Document 1005
 DOCN  M9471005
 TI    A spontaneous point mutation in the human T-cell leukemia virus type 1
       pX gene leads to expression of a novel doubly spliced pX-mRNA that
       encodes a 25-kD, amino-terminal deleted rex protein.
 DT    9409
 AU    Orita S; Kobayashi H; Saiga A; Kubota R; Osame M; Igarashi H; Shionogi
       Institute for Medical Science, Osaka, Japan.
 SO    DNA Cell Biol. 1994 Apr;13(4):353-64. Unique Identifier : AIDSLINE
       GENBANK/D13005
 AB    Primary RNA transcripts of the human T-cell leukemia virus type 1
       (HTLV-1) are processed into mature mRNA by a complex series of splicing
       events. In this paper, we report the finding of a novel doubly spliced
       pX mRNA in two out of eight HTLV-1-infected cell lines and in one out of
       13 peripheral blood mononuclear cells from HTLV-1-infected individuals.
       The second splicing for this novel pX mRNA is different from that for
       the known doubly spliced pX mRNA. A novel acceptor site in this splicing
       was generated by a single point mutation (G to A) at nucleotide 7,337 of
       the pX gene. This mRNA contained a complete open reading frame that
       encodes an amino-terminal truncated p27rex protein with 189 amino acids.
       A new 25-kD protein was detected in the cell lines expressing the novel
       pX mRNA by an antibody against the carboxy-terminal peptide of p27rex
       and was termed p25rex. Although the function of p25rex is not clear, we
       clarified that p25rex is a cytoplasmic phosphoprotein and its function
       is different from the transcriptional regulator function of p27rex. The
       possibility that the mutated virus is replicable only in cells
       coinfected with the wild type HTLV-1 may explain why the incidence of
       the mutants observed here is low.
 DE    Amino Acid Sequence  Base Sequence  Cell Line  DNA, Viral  Gene
       Products, rex/*GENETICS  Gene Products, tax/GENETICS  Human
       HTLV-I/*GENETICS  HTLV-I Infections/MICROBIOLOGY  Leukocytes,
       Mononuclear/MICROBIOLOGY  Molecular Sequence Data  *Point Mutation  *RNA
       Splicing  RNA, Messenger/*GENETICS  RNA, Viral/*GENETICS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).