Document 1066
 DOCN  M9471066
 TI    Cationic liposomes for direct gene transfer in therapy of cancer and
       other diseases (Meeting abstract).
 DT    9409
 AU    Huang L; Gao X; Farhood H; Son K; Dept. of Pharmacology, Univ. of
       Pittsburgh Sch. of Medicine,; Pittsburgh, PA
 SO    Gene Therapy for Neoplastic Diseases. June 26-29, 1993, Washington, DC,
       A5, 1993.. Unique Identifier : AIDSLINE ICDB/94698152
 AB    Cationic liposomes can mediate efficient delivery of DNA and DNA/protein
       complex to mammalian cells in vitro and in vivo. Cationic cholesterol
       derivatives mixed with phosphatidylethanolamine and sonicated to form
       small unilamellar vesicles can complex with DNA and mediate the entry
       into the cytosol from the endosome compartment. One of the liposome
       formulations, ie, DC-Chol liposomes, are used in a gene therapy clinical
       trial for melanoma. Recently, we exploited these cationic liposomes for
       the delivery of trans-activating protein factors to regulate and control
       the expression of delivered transgenes in a protein-dose dependent
       manner. Bacteriophage T7 RNA polymerase was co-delivered with a reporter
       gene under the control of T7 promoter to allow cytoplasmic expression of
       the gene. Human immunodeficiency virus-1 (HIV) trans-activating protein
       (tat) was also co-delivered with a reporter gene under the control of
       HIV-1 long terminal repeat. Finally, human tumor cells selected for
       cisplatin resistance, or isolated from patients who have failed
       cisplatin therapy are highly transfectable with cationic liposomes.
       These results suggest a serial therapy protocol with cisplatin and gene
       therapy for malignancy.
 DE    Animal  Cations  Clinical Trials  Gene Products, tat/GENETICS  *Gene
       Therapy  *Gene Transfer  Human  *Liposomes  Melanoma/THERAPY
       Neoplasms/*THERAPY  Phospholipids  RNA Polymerases/GENETICS  Tumor
       Cells, Cultured  MEETING ABSTRACT

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       protected by Copyright Law (Title 17, U.S.Code).