Document 0013
 DOCN  M9480013
 TI    Cationic liposomes for direct gene transfer in therapy of cancer and
       other diseases.
 DT    9410
 AU    Farhood H; Gao X; Son K; Yang YY; Lazo JS; Huang L; Barsoum J; Bottega
       R; Epand RM; Department of Pharmacology, University of Pittsburgh School
       of; Medicine, Pennsylvania 15261.
 SO    Ann N Y Acad Sci. 1994 May 31;716:23-34; discussion 34-5. Unique
       Identifier : AIDSLINE MED/94295994
 AB    Cationic liposomes can mediate efficient delivery of DNA and DNA/protein
       complex to mammalian cells in vitro and in vivo. Cationic cholesterol
       derivatives mixed with phosphatidylethanolamine and sonicated to form
       small unilamellar vesicles can complex with DNA and mediate the entry
       into the cytosol from the endosome compartment. One of the liposome
       formulations, DC-Chol liposomes, is used in a gene therapy clinical
       trial for melanoma. Recently, we exploited these cationic liposomes for
       the delivery of trans-activating protein factors to regulate and control
       the expression of delivered transgenes in a protein dose-dependent
       manner. Bacteriophage T7 RNA polymerase was co-delivered with a reporter
       gene under the control of T7 promoter to allow cytoplasmic expression of
       the gene. Human immunodeficiency virus-1 transactivating protein was
       also codelivered with a reporter gene under the control of HIV-1 long
       terminal repeat. Finally, human tumor cells selected for cis-platin
       resistance or isolated from patients who have failed cis-platin therapy
       are highly transfectable with cationic liposomes. These results suggest
       a serial therapy protocol with cis-platin and gene therapy for
       malignancy.
 DE    Animal  Cations  Cell Line  Cholesterol/CHEMISTRY
       Cisplatin/PHARMACOLOGY  Drug Resistance  Gene Expression Regulation
       *Gene Transfer  Genes, tat  Genetic Vectors  Human  HIV-1/GENETICS
       Liposomes  Neoplasms/*THERAPY  RNA Polymerases/GENETICS
       Structure-Activity Relationship  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE  REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).