Document 0028
 DOCN  M9480028
 TI    [Report of initial experiences in establishing a regional multicenter
       evaluation of lymphocyte typing with flow cytometry]
 DT    9410
 AU    Schonwald E; Jilch R; Serodiagnostische Station der Dermatologischen
       Abteilung des; Krankenhauses der Stadt Wien-Lainz.
 SO    Wien Klin Wochenschr. 1994;106(8):247-9. Unique Identifier : AIDSLINE
       MED/94295210
 AB    Participation in a multicenter evaluation of flowcytometric
       investigations is the only possibility of providing external quality
       control. Up to now nine different laboratories have taken part in this
       program, measuring two samples of fresh EDTA blood according to a
       recommended antibody panel. The choice of antibodies was intended to
       reach a standardized immune status. Three different types of
       flowcytometers were used. All results were expressed in lymphocyte
       subset percentages and in absolute values. The coefficient of variation
       of each lymphocyte subpopulation was used for interpretation of the
       results of all working groups, as well as the control measurement. The
       results of the control measurement already showed that there was a
       difference between the obtainable coefficient of variation for the
       different subpopulations. In the case of CD3, CD4 and CD8 positive
       lymphocytes the relative percentages of the total group gave values far
       below 10%. The coefficients of variation of the activated T-lymphocytes
       or the cytotoxic T-cells, as well as of the absolute values were
       definitely higher. Already after the second multicenter evaluation the
       achieved results showed positive aspects such as improvements of the
       outcome, advancement of the user contacts, cooperation and impulses by
       discussing interesting problems.
 DE    Antigens, CD/ANALYSIS  B-Lymphocyte Subsets/*IMMUNOLOGY  Comparative
       Study  CD4-CD8 Ratio  English Abstract  Flow Cytometry/*INSTRUMENTATION
       Human  HLA-DR Antigens/ANALYSIS  Immunophenotyping/*INSTRUMENTATION
       Killer Cells, Natural/IMMUNOLOGY  Leukocyte Count  Quality Control
       T-Lymphocyte Subsets/*IMMUNOLOGY  T-Lymphocytes, Cytotoxic/IMMUNOLOGY
       JOURNAL ARTICLE  MULTICENTER STUDY

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