Document 0052
 DOCN  M9480052
 TI    Lack of evidence for the dichotomy of TH1 and TH2 predominance in
       HIV-infected individuals [see comments]
 DT    9410
 AU    Graziosi C; Pantaleo G; Gantt KR; Fortin JP; Demarest JF; Cohen OJ;
       Sekaly RP; Fauci AS; Laboratory of Immunoregulation, National Institute
       of Allergy and; Infectious Diseases, National Institutes of Health,
       Bethesda, MD; 20892.
 SO    Science. 1994 Jul 8;265(5169):248-52. Unique Identifier : AIDSLINE
       MED/94294789
 CM    Comment in: Science 1994 Jul 8;265(5169):193-4
 AB    A switch from a T helper 1 (TH1) cytokine phenotype to a TH2 phenotype
       has been proposed as a critical element in the progression of human
       immunodeficiency virus (HIV) disease. Here, constitutive cytokine
       expression was analyzed in unfractionated and sorted cell populations
       isolated from peripheral blood and lymph nodes of HIV-infected
       individuals at different stages of disease. Expression of interleukin-2
       (IL-2) and IL-4 was barely detectable (or undetectable) regardless of
       the stage of disease. CD8+ cells expressed large amounts of interferon
       gamma and IL-10, and the levels of these cytokines remained stably high
       throughout the course of infection. Furthermore, similar patterns of
       cytokine expression were observed after stimulation in vitro of purified
       CD4+ T cell populations obtained from HIV-infected individuals at
       different stages of disease. These results indicate that a switch from
       the TH1 to the TH2 cytokine phenotype does not occur during the
       progression of HIV disease.
 DE    Antigens, CD8/ANALYSIS  Apoptosis  Cell Separation  Comparative Study
       Cross-Sectional Studies  Human  HIV Infections/*IMMUNOLOGY  Interferon
       Type II/*BIOSYNTHESIS  Interleukin-10/BIOSYNTHESIS
       Interleukin-2/BIOSYNTHESIS  Interleukin-4/BIOSYNTHESIS
       Interleukins/*BIOSYNTHESIS  Longitudinal Studies  Lymph Nodes/IMMUNOLOGY
       Lymphocyte Transformation  Phenotype  T-Lymphocyte
       Subsets/CYTOLOGY/*IMMUNOLOGY  T-Lymphocytes, Helper-Inducer/*IMMUNOLOGY
       JOURNAL ARTICLE

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