Document 0105
 DOCN  M9480105
 TI    Clinical and immunological correlates of immune-complex-dissociated
       HIV-1 p24 antigen in HIV-1-infected children.
 DT    9410
 AU    Duiculescu DC; Geffin RB; Scott GB; Scott WA; Victor Babes Hospital for
       Infectious Diseases, Bucharest,; Romania.
 SO    J Acquir Immune Defic Syndr. 1994 Aug;7(8):807-15. Unique Identifier :
       AIDSLINE MED/94293162
 AB    It has been reported that HIV-1 p24 antigen (p24 Ag) detection is
       improved after dissociation of immune complexes using acid treatment
       (ICD assay). In order to evaluate the clinical significance of p24 Ag
       detected by the standard assay and by the ICD assay in pediatric
       patients, we related these measurements to clinical status, level of p24
       antibody, and percentage of CD4+ lymphocytes. Fifty-nine plasma
       specimens from 20 symptomatic HIV-1-infected children, collected
       prospectively over a 1-year period, were tested for these markers.
       Plasma was collected at the beginning of zidovudine therapy and
       approximately 7 and 12 months thereafter. Compared with the standard
       assay, the ICD assay showed a higher number of samples positive for p24
       Ag (78% versus 34%) and an increase in the levels of p24 Ag (median
       value of 129 versus 24 pg/ml). The anti-p24 antibody level was inversely
       correlated with the p24 Ag level measured by either assay. Four children
       negative for p24 Ag by both assays had a stable clinical course. In
       contrast, 50% of the children negative by the standard assay but
       positive for ICD p24 Ag and 75% of the children positive by both assays
       had progression of disease. No patients were positive by the standard
       assay but negative by the ICD assay. Children whose plasma tested
       positive by both assays had lower percentages of lymphocytes that were
       CD4+ by comparison with children who were negative by both assays;
       children whose plasma tested positive only by the ICD assay formed an
       intermediate group.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Antigen-Antibody Complex/*METABOLISM  Child  Child, Preschool
       Comparative Study  Enzyme-Linked Immunosorbent Assay  Female  Follow-Up
       Studies  Human  HIV Antibodies/BLOOD  HIV Core Protein
       p24/*BLOOD/IMMUNOLOGY  HIV Infections/DRUG THERAPY/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Infant  Leukocyte Count  Male  Neutralization Tests
       Prognosis  Prospective Studies  Retrospective Studies  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  T4 Lymphocytes
       Zidovudine/THERAPEUTIC USE  JOURNAL ARTICLE

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