Document 0108
 DOCN  M9480108
 TI    Quantifying HIV-1 proviral DNA using the polymerase chain reaction on
       cerebrospinal fluid and blood of seropositive individuals with and
       without neurologic abnormalities.
 DT    9410
 AU    Schmid P; Conrad A; Syndulko K; Singer EJ; Handley D; Li X; Tao G;
       Fahy-Chandon B; Tourtellotte WW; Neurology Service, DVA Medical Center
       West Los Angeles, Wadsworth; Division, California.
 SO    J Acquir Immune Defic Syndr. 1994 Aug;7(8):777-88. Unique Identifier :
       AIDSLINE MED/94293159
 AB    To quantify the number of human immunodeficiency virus type 1 (HIV-1)
       proviral copies per 1,000 CD4+ cells in cerebrospinal fluid (CSF) and
       blood in relationship to stage of infection and HIV-1 neurologic disease
       (HND), 87 HIV-1 seropositive men without CNS opportunistic infections,
       tumors, or neurosyphilis, 9 high-risk, and 14 not-at-risk seronegative
       controls underwent a structured interview, and physical and neurologic
       examination followed by blood and CSF collection. A custom-designed,
       fully automated polymerase chain reaction (PCR) system performed
       amplification with use of two HIV-1 gag primer pairs, Southern blotting,
       and hybridization with product-specific probes. Image analysis was used
       to quantify band intensities relative to a dilution series. Eighty-one
       of 87 (93%) seropositive patients, 1 of 9 high-risk patients, (11%) and
       none of 14 seronegative controls had PCR-detectable HIV-1 in their
       blood. Fifty-seven of 63 (90%) seropositive patients, 2 of 5 (40%)
       high-risk seronegative patients, and none of 14 controls had HIV-1 in
       their CSF. The proviral load in seropositive patients, all stages, was
       significantly greater in CSF than blood [median 25 vs. 0.6 copies/1,000
       CD4+ cells (p = 0.0001)]. The median proviral load in blood was 0.09
       copies/1,000 CD4+ cells in seropositive, asymptomatic subjects, 10.7 in
       patients with AIDS, and 1.4 in patients with AIDS-related complex (p =
       0.0281). CSF proviral load was greater in seropositive patients with HND
       than those without HND, median 43.5 vs. 17.6 copies/1,000 CD4+ cells (p
       = 0.0614). Proviral load was greater in the blood and CSF of subjects
       with more advanced systemic disease and HND. There was a substantial
       penetration of HIV-1 into the CNS/CSF in both systemically and
       neurologically asymptomatic HIV-1 disease.
 DE    Adult  AIDS Dementia Complex/BLOOD/CEREBROSPINAL FLUID/DIAGNOSIS  Base
       Sequence  Blotting, Southern  Cerebrospinal Fluid/CYTOLOGY/MICROBIOLOGY
       DNA Primers/CHEMISTRY  DNA, Viral/*BLOOD/*CEREBROSPINAL FLUID  Human
       HIV Infections/BLOOD/CEREBROSPINAL FLUID/*DIAGNOSIS
       HIV-1/*GENETICS/ISOLATION & PURIF  Male  Molecular Sequence Data
       Polymerase Chain Reaction  Prognosis  Proviruses/*GENETICS/ISOLATION &
       PURIF  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  T4
       Lymphocytes/MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).