Document 0115
 DOCN  M9480115
 TI    Antiproliferative factor against the human glioblastoma cell line T98G
       identified in culture supernatants of CD4+ cells from patients with
       HTLV-I-associated myelopathy.
 DT    9410
 AU    Nishiura Y; Nakamura T; Ichinose K; Nagasato K; Ohishi K; Watanabe H;
       Shirabe S; Tsujihata M; Nagataki S; First Department of Internal
       Medicine, Nagasaki University School; of Medicine, Japan.
 SO    J Neurol Sci. 1994 Apr;122(2):214-9. Unique Identifier : AIDSLINE
       MED/94293037
 AB    We investigated culture supernatants of peripheral blood mononuclear
       cells (MNC) derived from patients with human T-lymphotropic virus type I
       (HTLV-I)-associated myelopathy (HAM) for antiproliferative activity
       against the human glioblastoma cell line T98G. When T98G cells were
       cultured with condition medium containing culture supernatants of MNC
       from patients with HAM, the proliferation of T98G cells was
       significantly suppressed, compared with that of supernatants from HTLV-I
       seropositive carriers or seronegative controls. To clarify which
       population of MNC produced the antiproliferative humoral factor for T98G
       cells, we separated MNC into macrophage-depleted or B cell depleted
       populations, and further to both CD4+ and CD8+ T cells by using the
       panning method or plastic adherence. These studies demonstrated that the
       antiproliferative activity was mediated by a humoral factor produced by
       T cells, specifically CD4+ cells. This activity was blocked by a
       neutralizing monoclonal antibody against interferon-gamma (IFN-gamma).
       Moreover, IFN-gamma levels were elevated in the culture supernatants of
       CD4+ cells from HAM patients. Thus, the antiproliferative activity
       against T98G cells is mainly due to IFN-gamma derived from CD4+ cells of
       patients with HAM.
 DE    Adult  Aged  Antibodies, Monoclonal/IMMUNOLOGY  Brain
       Neoplasms/*PATHOLOGY  Cell Division/DRUG EFFECTS  Cells, Cultured
       Culture Media, Conditioned/CHEMISTRY  Female  Glioblastoma/*PATHOLOGY
       Growth Inhibitors/*ISOLATION & PURIF/PHARMACOLOGY/SECRETION  Human
       Interferon Type II/IMMUNOLOGY/ISOLATION & PURIF/*PHARMACOLOGY/
       SECRETION  Male  Middle Age  Paraparesis, Tropical Spastic/*BLOOD
       Support, Non-U.S. Gov't  Tumor Cells, Cultured/DRUG EFFECTS  T4
       Lymphocytes/*SECRETION  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).