Document 0121
 DOCN  M9480121
 TI    DNA found in human immunodeficiency virus type 1 particles may not be
       required for infectivity.
 DT    9410
 AU    Arts EJ; Mak J; Kleiman L; Wainberg MA; Lady Davis Institute, Jewish
       General Hospital, Montreal,; Quebec, Canada.
 SO    J Gen Virol. 1994 Jul;75 ( Pt 7):1605-13. Unique Identifier : AIDSLINE
       MED/94292911
 AB    We have studied the presence and significance of retroviral
       genome-derived DNA in the core of human immunodeficiency virus (HIV)
       particles produced from transfections of HXB2 expression vectors in
       COS-7 cells and from HIV type 1 IIIB chronically infected H9 cells.
       Viruses purified by sucrose cushion centrifugation and treated with
       DNase I contained 1000-fold more viral RNA than DNA. However,
       protease-defective viruses that contained only p160gag-pol had less than
       100 times the amount of DNA in their cores than wild-type viruses
       suggesting that the p66/p51 form of reverse transcriptase was
       responsible for DNA transcription. Viruses produced by transfections in
       the presence of 3'-azido-3'-deoxythymidine (AZT) contained the viral RNA
       genome but only DNA of premature length because of the chain terminating
       effects of AZT. However such viruses were as infections for CD4+ cells
       as wild-type virus. We conclude that retrovirus-derived DNA in HIV-1
       particles is not required for infection and does not play a significant
       role in this process.
 DE    Animal  Cell Line  DNA, Viral/*ANALYSIS  Gene Amplification  Gene
       Products, gag/PHYSIOLOGY  *Genome, Viral  Human  HIV Core Protein
       p24/ANALYSIS  HIV-1/CHEMISTRY/*GENETICS/PHYSIOLOGY  Polymerase Chain
       Reaction  Protein Precursors/PHYSIOLOGY  Reverse
       Transcriptase/PHYSIOLOGY  RNA, Viral/ANALYSIS/GENETICS  Support,
       Non-U.S. Gov't  T-Lymphocytes/CYTOLOGY  Transfection  Virus
       Replication/DRUG EFFECTS  Zidovudine/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).