Document 0122
 DOCN  M9480122
 TI    Definition of the range and distribution of human immunodeficiency virus
       macrophage tropism using PCR-based infectivity measurements.
 DT    9410
 AU    Collin M; Illei P; James W; Gordon S; Sir William Dunn School of
       Pathology, University of Oxford, U.K.
 SO    J Gen Virol. 1994 Jul;75 ( Pt 7):1597-603. Unique Identifier : AIDSLINE
       MED/94292910
 AB    The tropism of human immunodeficiency virus (HIV) for macrophages (m
       phi) is a well recognized phenomenon, but the range and distribution of
       m phi-tropic phenotypes have not been defined by quantitative means.
       This study uses a PCR-based infectivity assay to derive an index of m
       phi tropism for several common strains of HIV. The results show that m
       phi tropism varies over about six orders of magnitude and that the most
       m phi-tropic strains have a higher infectivity for m phi than for
       peripheral blood lymphocytes. Strains were distributed throughout this
       range, suggesting that m phi tropism is a continuously variable
       phenotypic property. Although the degree of tropism was strongly
       influenced by the mode of isolation and propagation of virus strains,
       there was no evidence for the existence of distinct m phi-tropic or
       non-m phi-tropic phenotypes. Finally, the tropism of two selected
       strains was found to be determined by an early step in replication,
       probably virus entry.
 DE    Base Sequence  Cell Line  Comparative Study  Human
       HIV/GENETICS/IMMUNOLOGY/*PHYSIOLOGY  HIV Core Protein p24/ANALYSIS
       Macrophage Activation  Macrophages/IMMUNOLOGY/*MICROBIOLOGY  Molecular
       Sequence Data  *Polymerase Chain Reaction  Species Specificity  Support,
       Non-U.S. Gov't  T-Lymphocytes/MICROBIOLOGY  Virus Replication  JOURNAL
       ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).