Document 0125
 DOCN  M9480125
 TI    Projection structures of human immunodeficiency virus type 1 (HIV-1)
       observed with high resolution electron cryo-microscopy.
 DT    9410
 AU    Goto T; Ashina T; Fujiyoshi Y; Kume N; Yamagishi H; Nakai M; Department
       of Microbiology, Osaka Medical College, Japan.
 SO    J Electron Microsc (Tokyo). 1994 Feb;43(1):16-9. Unique Identifier :
       AIDSLINE MED/94292879
 AB    An electron microscopical study was made of human immunodeficiency virus
       type 1 (HIV-1) in the unstained, frozen, hydrated state at liquid helium
       temperature. The findings were compared with those obtained from
       negative staining and ultrathin sectioning. Electron cryo-microscopy
       confirms and extended the findings obtained by conventional methods. In
       particular, the virus is shown to be globular, the lipid membrane is
       clearly resolved as a bi-layer and the bi-layer was demonstrated to be
       non-uniform in width. The projections studied in the bi-layer are
       clearly observed to be knob-like and consist of a head, a stalk and a
       base.
 DE    Freezing  HIV-1/*ULTRASTRUCTURE  Membranes/ULTRASTRUCTURE  Microscopy,
       Electron/*METHODS  Support, Non-U.S. Gov't  Viral Envelope
       Proteins/*ULTRASTRUCTURE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).