Document 0153
 DOCN  M9480153
 TI    Establishment and characterization of synovial cell clones with
       integrated human T-cell leukemia virus type-I.
 DT    9410
 AU    Hasunuma T; Nakajima T; Aono H; Sato K; Matsubara T; Yamamoto K;
       Nishioka K; Division of Rheumatology and Molecular Immunology, St.
       Marianna; University School of Medicine, Kanagawa-ken, Japan.
 SO    Clin Immunol Immunopathol. 1994 Jul;72(1):90-7. Unique Identifier :
       AIDSLINE MED/94291307
 AB    Human T cell leukemia virus type-I (HTLV-I)-integrated synovial cell
       clones (SCCs) were established from four HTLV-I-infected patients with
       chronic proliferative arthropathy. Extracted DNAs of these SCCs were
       examined for the existence of HTLV-I proviral DNA. Electron microscopic
       analysis and immunohistochemical staining were also performed to examine
       the nature of the HTLV-I integrated SCCs. SCCs derived from all four
       patients consisted of either HTLV-I integrated or nonintegrated cells.
       HTLV-I integrated SCCs also produced viral messenger RNA which was
       detected by reverse transcriptase-polymerase chain reaction as well as
       viral protein which was detected by immunohistochemical staining.
       Phenotypically, these SCCs containing HTLV-I proviral DNA were shown to
       be all cell types as observed by electron microscopic analysis and
       immunohistochemical analysis. Moreover, expression of HTLV-I
       glycoprotein was greater in SCCs with a monocyte marker. These findings
       suggest that SCCs harboring HTLV-I have an important role in synovial
       hyperplasia.
 DE    Aged  Aged, 80 and over  Clone Cells  DNA, Viral/*ANALYSIS  Female
       Human  HTLV-I/*GENETICS/ISOLATION & PURIF  Immunohistochemistry  Middle
       Age  Proviruses/GENETICS/ISOLATION & PURIF  RNA, Messenger/ANALYSIS
       RNA, Viral/ANALYSIS  Support, Non-U.S. Gov't  Synovial
       Membrane/*CYTOLOGY/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).