Document 0181
 DOCN  M9480181
 TI    Interleukin-2-dependent T-cell lines established from paroxysmal
       nocturnal hemoglobinuria patients.
 DT    9410
 AU    Nakakuma H; Nagakura S; Horikawa K; Hidaka M; Kawaguchi T; Iwamoto N;
       Sanada I; Kagimoto T; Takatsuki K; Second Department of Internal
       Medicine, Kumamoto University; School of Medicine, Japan.
 SO    Blood. 1994 Jul 1;84(1):309-14. Unique Identifier : AIDSLINE
       MED/94289717
 AB    Peripheral blood T lymphocytes obtained from two patients with
       paroxysmal nocturnal hemoglobinuria (PNH) were immortalized with human
       T-lymphotropic virus type 1 (HTLV-1). These cells showed interleukin-2
       (IL-2)-dependent cell growth in culture. Cell surface analysis showed
       that they had the phenotype of a helper/inducer T subset that was
       positive for CD2, CD3, and CD4, but negative for CD8, similar to adult
       T-cell leukemia cells induced by HTLV-1. These cell lines lacked
       glycosylphosphatidylinositol (GPI)-anchored proteins, CDw52, CD55
       (decay-accelerating factor; DAF), and CD59 on the cell surface, whereas
       intracellular DAF protein was detected. These T-subset cell lines with a
       PNH phenotype did not synthesize GPI anchor, whereas a control cell
       line, similarly prepared from the T cells of a healthy volunteer,
       produced the anchor. The control cells expressed CDw52, DAF, and CD59 on
       the cell surface and showed the phenotype of a helper/inducer subset.
       Southern blot analysis confirmed the clonality of each cell line. These
       CD4+ T-cell lines with a PNH phenotype and a subset-matched control
       counterpart could be a useful model for PNH investigation.
 DE    Antigens, CD/ANALYSIS  Cell Line
       Glycosylphosphatidylinositols/BIOSYNTHESIS  Hemoglobinuria,
       Paroxysmal/*IMMUNOLOGY  Human  HTLV-I/GENETICS
       Interleukin-2/*PHARMACOLOGY  Karyotyping  Lymphocyte Transformation/DRUG
       EFFECTS  Membrane Glycoproteins/ANALYSIS  Support, Non-U.S. Gov't
       T-Lymphocytes/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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