Document 0182
 DOCN  M9480182
 TI    Effect of human immunodeficiency virus type-1 envelope glycoprotein
       gp160 on cytokine production from cord-blood T cells.
 DT    9410
 AU    Than S; Oyaizu N; Pahwa RN; Kalyanaraman VS; Pahwa S; Department of
       Pediatrics, North Shore University Hospital-Cornell; University Medical
       College, Manhasset, NY 11030.
 SO    Blood. 1994 Jul 1;84(1):184-8. Unique Identifier : AIDSLINE MED/94289699
 AB    We have recently demonstrated that the human immunodeficiency virus type
       1 (HIV-1) envelope glycoprotein gp160 enhances the in vitro
       differentiation of hematopoietic myeloid progenitor cells derived from
       cord blood by inducing secretion of colony-stimulating factor(s) (CSF)
       in T cells, presumably through the interaction of gp160 with CD4
       molecules. In this study, we investigated the gp 160-induced humoral
       CSFs in cord blood by enzyme-linked immunosorbent assay (ELISA) and by
       polymerase chain reaction on reverse-transcribed mRNA (RT-PCR). We
       demonstrate that gp160 can induce interleukin (IL)-3, IL-6, and
       granulocyte-macrophage CSF (GM-CSF) protein secretion only in purified
       cord-blood T cells (CB-T) and not in detectable amounts in whole cord
       blood cells (WCB); cytokine mRNA induction occurred in purified CB-T and
       WCB, but was significantly greater in the former. Treatment of gp160
       with soluble CD4 (sCD4) abolished the secretion of all three cytokines
       in CB-T cells, which suggests that interaction of gp160 with CD4
       molecules is required for the secretion of these cytokines from CB-T
       cells. However, in WCB cells, sCD4 treatment of gp160 resulted in
       inhibition of only IL-3 and GM-CSF mRNA, whereas IL-6 secretion was
       enhanced. Purified cord-blood monocytes secreted only IL-6 in response
       to gp160, and the gp160-induced IL-6 secretion by monocytes was also
       further increased by gp160 + sCD4 complex. Furthermore, monocyte culture
       supernatants suppressed gp160-induced IL-3 secretion from CB-T cells.
       These findings indicate that (1) CB-T cells are a potent source of
       gp160-induced hematopoietic cytokines, and (2) that different mechanisms
       are involved in the induction of IL-6 by gp160 in the T- and non-T-cell
       fractions of cord blood. The ability of HIV gp160 to induce
       hematopoietic CSFs in cord blood may be important in HIV pathogenesis.
 DE    Antigens, CD4/PHYSIOLOGY  Antigens, CD45/ANALYSIS  Base Sequence
       Cytokines/*BIOSYNTHESIS  Fetal Blood/*METABOLISM  Gene Products,
       env/*PHARMACOLOGY  Granulocyte-Macrophage Colony-Stimulating
       Factor/BIOSYNTHESIS/  GENETICS  Human  *HIV-1  Infant, Newborn
       Interleukin-3/BIOSYNTHESIS/GENETICS  Interleukin-6/BIOSYNTHESIS/GENETICS
       Molecular Sequence Data  Protein Precursors/*PHARMACOLOGY  RNA,
       Messenger/ANALYSIS  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).