Document 0204
 DOCN  M9480204
 TI    SIVmac expressing hybrid envelope proteins containing HIV-1 V3 and/or C4
       sequences is not competent for replication.
 DT    9410
 AU    Kirchhoff F; Morrison HG; Murray MG; Rennert P; Desrosiers RC; New
       England Regional Primate Research Center, Harvard Medical; School,
       Southborough, Massachusetts 01772-9102.
 SO    AIDS Res Hum Retroviruses. 1994 Mar;10(3):309-13. Unique Identifier :
       AIDSLINE MED/94289070
 AB    The V3- and C4-coding regions in the envelope gene of the infectious,
       pathogenic SIVmac239 clone were replaced by the corresponding HIV-1
       sequences. Viral particles were obtained after transfection of COS-1
       cells. Chimeric SIVmac constructs were not replication competent in the
       human T cell lines CEMx174, AA2, H9, and MT-4 or in primary cultures of
       rhesus monkey peripheral blood mononuclear cells. The lack of
       infectivity of the hybrid constructs was associated with inefficient
       proteolytic processing of the gp160env precursor. Unlike the modular
       nature of some proteins, gp120 appears to be a highly ordered molecule
       whose function is dependent on the integration of many discontinuous,
       interactive regions.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cell Line  DNA, Viral  Human
       HIV Envelope Protein gp120/*GENETICS  HIV-1/GENETICS/*PHYSIOLOGY
       Molecular Sequence Data  Peptide Fragments/GENETICS
       Radioimmunoprecipitation Assay  Recombinant Fusion Proteins/GENETICS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       SIV/GENETICS/IMMUNOLOGY/*PHYSIOLOGY  Transfection  Viral Envelope
       Proteins/*BIOSYNTHESIS/GENETICS/IMMUNOLOGY  Virus
       Replication/GENETICS/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).