Document 0206
 DOCN  M9480206
 TI    A sensitive reporter cell line for HIV-1 tat activity, HIV-1 inhibitors,
       and T cell activation effects.
 DT    9410
 AU    Aguilar-Cordova E; Chinen J; Donehower L; Lewis DE; Belmont JW;
       Institute for Molecular Genetics, Baylor College of Medicine,; Houston,
       Texas 77030.
 SO    AIDS Res Hum Retroviruses. 1994 Mar;10(3):295-301. Unique Identifier :
       AIDSLINE MED/94289068
 AB    The production and characterization of a cell line for quantitative
       HIV-1 Tat function and T cell activation assays is described. 1G5 is a
       clonal cell line derived from Jurkat T cells stably transfected with a
       luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The
       1G5 clone was selected for low basal luciferase activity, susceptibility
       to HIV infection, and high responsiveness to Tat and T cell activation
       signals. A 10 to 1000-fold increase in luciferase activity after
       transfection or infection with tat-expressing vectors or HIV was
       observed. Equivalent levels of expression were detected after
       stimulation with T cell mitogens. The characteristics of 1G5 make it a
       valuable reagent for studies of HIV infection, HIV regulatory agents,
       and other T cell or HIV-activating factors, and for screening potential
       anti-HIV therapeutic agents.
 DE    Amino Acid Sequence  *Cell Line  DNA Primers  Gene Products,
       tat/*METABOLISM  Genes, tat  Human  HIV-1/DRUG EFFECTS/*PHYSIOLOGY
       Luciferase/GENETICS/METABOLISM  Lymphocyte Transformation  Molecular
       Sequence Data  Support, U.S. Gov't, P.H.S.  T-Lymphocytes/DRUG
       EFFECTS/IMMUNOLOGY/*MICROBIOLOGY  Zidovudine/PHARMACOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).