Document 0207
 DOCN  M9480207
 TI    Defective infection of rabbit peripheral blood monocyte cultures with
       human immunodeficiency virus type 1.
 DT    9410
 AU    Tseng CK; Leibowitz J; Sell S; Department of Pathology and Laboratory
       Medicine, Medical School,; University of Texas Health Science Center at
       Houston 77225.
 SO    AIDS Res Hum Retroviruses. 1994 Mar;10(3):285-93. Unique Identifier :
       AIDSLINE MED/94289067
 AB    Human immunodeficiency virus type 1 (HIV-1) produces abortive infections
       of primary cultures of rabbit peripheral blood mononuclear cells
       (PMBCs). Mitogen activation of rabbit PBMCs or the addition of exogenous
       cytokines to the cultures does not change the level of release of the
       early HIV core protein, p24, into the culture medium. The amount of p24
       increases steadily and reaches peak levels about 7 days after infection.
       HIV-1-specific DNA env sequences are also detected in infected PBMCs.
       However, reverse transcription of RNA of samples from activated infected
       cultures to cDNA, followed by amplification by the polymerase chain
       reaction, revealed transcription of gag, but not env, regions,
       suggesting that HIV-1 infection of rabbit PBMC does not lead to the
       replication and maturation of complete HIV-1 virions. In addition,
       neither CPE nor lytic infection was observed in HIV-1-infected rabbit
       cells and infectivity could not be transferred from rabbit cells
       infected with HIV for up to 2 weeks to MT-2 or H9 indicator cell lines.
       In addition, no CPE was seen in long-term cultures of the
       HTLV-I-transformed rabbit cell line PLT-1441, after inoculation with
       HIV. It is concluded that primary rabbit PBMCs may be infectable by
       HIV-1 but are not permissive for production of infectious virus. This
       conclusion is consistent with the apparent long-term latent infection
       seen after inoculation of rabbits with HIV-1 or HIV-1-infected cells.
 DE    Animal  Cell Line  Cells, Cultured  Cytopathogenic Effect, Viral
       Defective Viruses  Human  HIV Core Protein p24/BIOSYNTHESIS
       HIV-1/*PHYSIOLOGY  Kinetics  Leukocytes, Mononuclear/*MICROBIOLOGY
       Rabbits  Support, Non-U.S. Gov't  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).