Document 0208
 DOCN  M9480208
 TI    Quantitative assessment of human immunodeficiency virus type 1
       replication in human xenografts of acutely infected Hu-PBL-SCID mice.
 DT    9410
 AU    Koup RA; Hesselton RM; Safrit JT; Somasundaran M; Sullivan JL; Aaron
       Diamond AIDS Research Center, New York University School of; Medicine,
       New York 10016.
 SO    AIDS Res Hum Retroviruses. 1994 Mar;10(3):279-84. Unique Identifier :
       AIDSLINE MED/94289066
 AB    Replication of the IIIB strain of human immunodeficiency virus type 1
       (HIV-1IIIB) in CB.17 scid/scid mice reconstituted with human peripheral
       blood lymphocytes (Hu-PBL-SCID) was investigated. Hu-PBL-SCID mice could
       be infected, in a dose-dependent manner, when HIV-1IIIB was injected
       intraperitoneally. Replication-competent HIV-1 could be recovered from
       spleen, peripheral blood, bone marrow, thymus, lymph node, and
       peritoneal cavity, indicating the ability of the infection to spread to
       human tissue throughout the chimeric animals. HIV-1 infection was
       quantitated using p24 determination, end-point dilution culture, and
       polymerase chain reaction amplification. The level of HIV-1 replication
       in Hu-PBL-SCID mice was found to approximate the level reported in human
       infection. No HIV-1-specific immune response was generated to this
       infection, but nonspecific immune activation did occur, as also reported
       in human infection. Similarities therefore exist between HIV-1 infection
       of humans and Hu-PBL-SCID mice, which makes the latter an in vivo model
       in which to study HIV-1 replication.
 DE    Animal  Human  HIV Infections/*MICROBIOLOGY  HIV-1/ISOLATION &
       PURIF/*PHYSIOLOGY  *Lymphocyte Transfusion  Mice  Mice, SCID  Models,
       Biological  Polymerase Chain Reaction  Support, Non-U.S. Gov't  Support,
       U.S. Gov't, P.H.S.  Transplantation, Heterologous  *Virus Replication
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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