Document 0209
 DOCN  M9480209
 TI    Genetic analysis of HIV-1 during rapid progression to AIDS in an
       apparently healthy man.
 DT    9410
 AU    Oka S; Ida S; Shioda T; Takebe Y; Kobayashi N; Shibuya Y; Ohyama K;
       Momota K; Kimura S; Shimada K; Department of Infectious Diseases,
       University of Tokyo, Japan.
 SO    AIDS Res Hum Retroviruses. 1994 Mar;10(3):271-7. Unique Identifier :
       AIDSLINE MED/94289065
 AB    We encountered a case of HIV-1 infection in a previously healthy man,
       which was characterized by rapid progression to AIDS and death within 7
       months in association with high levels of antigenemia throughout the
       clinical course and no humoral immune response for at least 6 months.
       Genetic changes of the third variable domain (V3) of the envelope gene
       of HIV-1 in serum samples were analyzed at four time points during his
       rapid clinical course. The nucleotide changes were confined to a maximum
       of three substitutions among 105 nucleotides of the V3 region. A major
       population of the viral clones in this patient showed one amino acid
       substitution from aspartic acid (a negatively charged amino acid) to
       lysine (a positively charged amino acid) at position 30 from the first
       cysteine of the V3 loop. This substitution was thought to be associated
       with phenotypic changes, and viruses with this sequence in the V3 region
       had a strong syncytium-inducing ability in MT-4 cells. It appears that
       the lack of a humoral immune response accelerated disease progression in
       our patient and a genetic change that appeared to produce a phenotypic
       change occurred at an early stage of the disease.
 DE    Acquired Immunodeficiency Syndrome/IMMUNOLOGY/*MICROBIOLOGY/
       *PHYSIOPATHOLOGY  Adult  Base Sequence  Case Report  Cell Line  DNA,
       Viral  Human  HIV Seropositivity/MICROBIOLOGY
       HIV-1/*GENETICS/IMMUNOLOGY  Male  Molecular Sequence Data  Polymerase
       Chain Reaction  Sequence Homology, Nucleic Acid  Support, Non-U.S. Gov't
       Time Factors  Variation (Genetics)  JOURNAL ARTICLE

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