Document 0210
 DOCN  M9480210
 TI    Biological, serological, and genetic characterization of HIV-1 subtype E
       isolates from northern Thailand.
 DT    9410
 AU    Ichimura H; Kliks SC; Visrutaratna S; Ou CY; Kalish ML; Levy JA;
       Department of Medicine, School of Medicine, University of; California,
       San Francisco 94143-0128.
 SO    AIDS Res Hum Retroviruses. 1994 Mar;10(3):263-9. Unique Identifier :
       AIDSLINE MED/94289064
 AB    Twenty-three HIV-1 isolates were recovered from PBMCs from 26
       HIV-1-seropositive individuals in northern Thailand. The viruses grew
       readily in human PBMCs but only 7 of 17 (41.2%) and 5 of 17 (29.4%)
       replicated and only at a low level in primary macrophages and in
       established T cell lines, respectively. By immunoblot assays, sera from
       Thai subjects were strongly reactive with gp120 from a Thailand isolate,
       moderately reactive with a Rwandan isolate, and weakly reactive with a
       North American strain. These three viruses represent, respectively,
       examples of subtypes E, A, and B as classified by the sequences of the
       envelope region. Serological assays indicated that broadly reactive
       rather than type-specific neutralizing activity was detected among these
       northern Thai sera. The majority of the sera (approximately 75%)
       neutralized a representative Thailand isolate and the Rwanda isolate but
       only 55% neutralized the North American strain. However, the difference
       was not statistically significant. The genetic analyses indicated that
       nearly all the Thai isolates were highly homogeneous and distinct from
       the North American/European consensus sequence (subtype B); they belong
       to subtype E. This is the first report providing biological,
       serological, and genetic characterization of HIV-1 strains from
       Thailand. The findings suggest these viruses were recently introduced
       into the country and that serological evaluation of viral strains needs
       to be considered along with genetic subtyping when developing an HIV-1
       vaccine.
 DE    Adult  Amino Acid Sequence  Base Sequence  Cell Line  DNA, Viral  Female
       Human  HIV Envelope Protein gp120/CHEMISTRY/IMMUNOLOGY  HIV
       Seropositivity/*MICROBIOLOGY  HIV-1/*CLASSIFICATION/ISOLATION & PURIF
       Immunoblotting  Male  Molecular Sequence Data  Peptide
       Fragments/CHEMISTRY/IMMUNOLOGY  Sequence Homology, Amino Acid
       Serotyping  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Thailand  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).