Document 0418
 DOCN  M9480418
 TI    IL-10 inhibits HIV-1 replication and is induced by tat.
 DT    9410
 AU    Masood R; Lunardi-Iskandar Y; Moudgil T; Zhang Y; Law RE; Huang CL; Puri
       RK; Levine AM; Gill PS; Department of Medicine, University of Southern
       California, Los; Angeles 90033.
 SO    Biochem Biophys Res Commun. 1994 Jul 15;202(1):374-83. Unique Identifier
       : AIDSLINE MED/94311907
 AB    Interleukin 10 (IL-10) is produced by TH2 lymphocytes and regulates both
       lymphoid and myeloid cells. In the present study we demonstrate that
       IL-10 is expressed and produced spontaneously in the peripheral blood
       mononuclear cells (PBMCs) of all HIV-1 infected individuals tested, 3 of
       19 cases of HIV-negative lymphoma and none of five healthy controls.
       IL-10 mRNA was detectable in both monocytes/macrophages and T
       lymphocytes isolated from PBMCs of HIV infected patients. We have also
       shown that infection of promonocytic (U937) and T (H9) cell lines with
       HIV stimulates IL-10 secretion. Furthermore, a T cell line (H9) stably
       transfected with a HIV tat expression-vector secreted higher levels of
       IL-10. We have also demonstrated that rhIL-10 inhibited HIV-1
       replication in infected monocytes and PBMCs in a dose dependent manner.
       IL-10 may thus participate in long latency between HIV-1 infection and
       development of AIDS.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*IMMUNOLOGY
       B-Lymphocytes/*IMMUNOLOGY/METABOLISM  Base Sequence  Cell Line  DNA
       Primers  Gene Expression  Gene Products, tat/*METABOLISM  Genes, tat
       Human  HIV Seronegativity  HIV-1/DRUG EFFECTS/*PHYSIOLOGY
       Interleukin-10/BIOSYNTHESIS/*PHARMACOLOGY/*PHYSIOLOGY
       Lymphoma/BLOOD/IMMUNOLOGY  Macrophages/IMMUNOLOGY  Molecular Sequence
       Data  Monocytes/IMMUNOLOGY/METABOLISM  Polymerase Chain Reaction/METHODS
       Reference Values  RNA, Messenger/ANALYSIS/BIOSYNTHESIS  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes/*IMMUNOLOGY/METABOLISM  Transfection  Virus
       Replication/DRUG EFFECTS/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).