Document 0443
 DOCN  M9480443
 TI    In vivo induction of human immunodeficiency virus type 1-specific
       cytotoxic T lymphocytes and delayed-type hypersensitivity by a 23-amino
       acid peptide from the highly conserved region in major core protein p24.
 DT    9410
 AU    Nishino Y; Kameoka M; Okada Y; Zhong Q; Kimura T; Azuma I; Ikuta K;
       Institute of Immunological Science, Hokkaido University, Sapporo,;
       Japan.
 SO    Vaccine. 1994 May;12(6):485-91. Unique Identifier : AIDSLINE
       MED/94310824
 AB    Cell-mediated immune responses are a major immune defence mechanism
       against the spread of human immunodeficiency virus type 1 (HIV-1) which
       may lead to acquired immune deficiency syndrome (AIDS). Therefore, the
       best candidate for a peptide vaccine preventive from the onset of the
       disease might be a chain section containing both B- and T-cell epitopes
       in regions of conserved sequences between the different HIV-1 isolates.
       We previously identified the highly conserved linear B-cell epitope (23
       amino acids in the major core protein p24). Since the epitopes of
       cytotoxic T lymphocytes (CTLs) can be defined by short synthetic
       peptides, we examined whether this highly conserved region can elicit
       viral-specific, cell-mediated immune responses. The results showed
       specific induction of CD8+ CTLs in mice by immunization with the Gag
       13-mer peptide. Lysis of targets is specific since unpulsed cells with
       the same MHC haplotype or cells with a different MHC haplotype pulsed
       with the peptide were resistant to lysis. This in vivo response induced
       by the Gag 23-mer peptide was almost the same as that induced by the
       15-amino acid peptide from the HIV-1 Env gp120 which is an
       immunodominant domain in the V3 loop. Lymphocyte proliferation of T-cell
       fraction from immune spleen cells was observed after in vitro
       stimulation with the Gag 23-mer peptide, whereas there was no apparent
       lymphocyte proliferation with the Env 15-mer peptide. In addition,
       specific antibodies were raised against Gag p24 in mice immunized with
       the Gag 23-mer peptide.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Amino Acid Sequence  Animal  Antigens, CD8/ANALYSIS  Cell Line  Guinea
       Pigs  Hypersensitivity, Delayed/*ETIOLOGY  HIV Core Protein
       p24/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Immunization  Lymphocyte
       Transformation  Mice  Mice, Inbred BALB C  Molecular Sequence Data
       Peptide Fragments/*IMMUNOLOGY  Support, Non-U.S. Gov't  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  T4 Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

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