Document 0819
 DOCN  M9480819
 TI    Quantitative determination of HIV-1 p24 antigen in HIV antibody positive
       sera following alkaline dissociation of immune complexes.
 DT    9410
 AU    Swack N; Ramirez M; Berberich S; Stapleton J; Hygienic Laboratory, Univ.
       of Iowa, Iowa City.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:624 (abstract no. V-37). Unique
       Identifier : AIDSLINE ASM94/94313126
 AB    Assays for the detection of HIV-1 antigen have been used for the
       detection of circulating virus early in infection and also for
       assessment of disease progression and response to antiviral therapy.
       However HIV-1 p24 antigen may be complexed with HIV antibody and thus
       escape detection by antigen capture immunoassay. Previous studies to
       assay virus after acid dissociation of the immune complexes was found to
       be time consuming and required large quantities of serum. These problems
       were significantly reduced by the use of alkaline dissociation which was
       significantly less labor intensive and required smaller amounts of
       serum. Ninety seven serum specimens obtained from patients at the
       University of Iowa AIDS Clinic were studied. All sera were found to be
       HIV-1 antibody positive by Western Blot analysis. The semiquantitative
       detection of p24 antigen was studied by antigen capture assays obtained
       from commercial sources (Abbott Laboratories and Organon Teknika).
       Antigen dissociation was performed with the Vironostika HIV-1 Antigen
       Base Dissociation Assay. Both antigen assays without dissociation found
       35% of the sera to be positive for the presence of p24 antigen.
       Following treatment with the alkaline dissociation reagents 67% of the
       same sera were positive for the presence of p24 antigen. All of these
       could be neutralized by specific antibody to HIV. Quantitative p24
       determinations performed by both the undissociation and alkaline
       dissociation procedures were compared with patient lymphocyte
       immunophenotyping studies.
 DE    Acquired Immunodeficiency Syndrome/*BLOOD/IMMUNOLOGY  Antigen-Antibody
       Complex/*BLOOD/CHEMISTRY/ISOLATION & PURIF  Blotting, Western/METHODS
       Comparative Study  Human  HIV Antibodies/*BLOOD  HIV Antigens/BLOOD  HIV
       Core Protein p24/*BLOOD  HIV-1/IMMUNOLOGY/*ISOLATION & PURIF
       Immunophenotyping/METHODS  Lymphocytes/IMMUNOLOGY  Reagent Kits,
       Diagnostic  MEETING ABSTRACT

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