Document 0821
 DOCN  M9480821
 TI    Susceptibility of mice with MAIDS to cytomegalovirus retinitis at times
       relative to Th1/Th2 shift.
 DT    9410
 AU    Dix RD; Cray C; Cousins SW; Bascom Palmer Eye Institute, University of
       Miami School of; Medicine, FL.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:623 (abstract no. V-32). Unique
       Identifier : AIDSLINE ASM94/94313124
 AB    Prior to absolute decline in CD4+ T cell number in late-stage AIDS,
       HIV-1 infection produces a relative immune dysregulation caused by a
       shift in CD4+ T helper (Th) function from a mechanism that promotes
       cell-mediated immunity (Th1 response) to one that favors humoral
       immunity (Th2 response). Theoretically, this dysregulation predisposes
       to opportunistic infections. Mice with retrovirus-induced
       immunodeficiency syndrome (MAIDS) also exhibit a Th1/Th2 shift that
       becomes evident approximately 3 wks after retrovirus infection. We
       therefore explored the susceptibility of mice with MAIDS to murine
       cytomegalovirus (MCMV) retinitis at times relative to Th1/Th2 shift.
       Groups of adult C57BL/6 mice were infected with a mixture of murine
       retroviruses (LP-BM5 MuLV); control animals received PBS. At 2 wks
       (MAIDS-2), 4 wks (MAIDS-4), and 12 wks (MAIDS-12), MuLV-infected and
       age-matched control mice were inoculated by subretinal injection with
       MCMV and compared 10 days postinfection for evidence of MCMV replication
       and typical retinitis. When compared with immunocompetent controls,
       MAIDS-4 and MAIDS-12 mice demonstrated increased ocular MCMV titers.
       Whereas the frequency of typical necrotizing retinitis in MAIDS-12
       animals was 88%, retinitis was not observed in immunocompetent, MAIDS-2
       or MAIDS-4 animals. Nonetheless, evidence of retinal infection without
       retinitis was observed in 0%, 12% and 70% of immunocompetent, MAIDS-2,
       and MAIDS-4 animals, respectively. We conclude that although typical
       MCMV retinitis cannot be induced at time of initial Th1/Th2 shift,
       increased MCMV replication and evidence of retinal infection is
       observed.
 DE    Animal  Antibody Formation  Comparative Study  Cytomegalovirus
       Retinitis/*IMMUNOLOGY  Disease Susceptibility  Immunity, Cellular  Mice
       Murine Acquired Immunodeficiency Syndrome/*COMPLICATIONS/  *IMMUNOLOGY
       Muromegalovirus  T-Lymphocytes, Helper-Inducer/*IMMUNOLOGY  Time Factors
       T4 Lymphocytes/*IMMUNOLOGY  MEETING ABSTRACT

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