Document 0827
 DOCN  M9480827
 TI    Evaluation of a human immunodeficiency virus-1 antibody immunoblot for
       the confirmation of oral specimens.
 DT    9410
 AU    Griffin CK; Chun JK; Allen NM; Gaudette ND; Erbling MC; Jacobs AL;
       Goldstein AS; Cimler BM; Epitope, Inc., Beaverton, Oregon.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:622 (abstract no. V-29). Unique
       Identifier : AIDSLINE ASM94/94313118
 AB    Western blot (WB) assays are considered the gold standard for confirming
       retroviral screening test results. Specimens obtained with the OraSure
       oral specimen collection device have been extensively tested for the
       detection of antibodies to HIV and other viral infections. An HIV-1 WB
       was developed for the confirmation of the immunostatus of OraSure
       specimens. To evaluate the performance of this WB, paired serum and oral
       samples collected from 226 seropositive and 204 seronegative subjects
       were tested. The serum specimens were tested using an FDA-licensed HIV-1
       WB kit; the matching OraSure specimens were tested using the OraSure WB
       assay. Among the 226 confirmed seropositive samples, the OraSure HIV-1
       WB was positive for 224 samples and indeterminate for the remaining two.
       No false negative samples were observed. None of the 204 specimens
       identified as HIV-1 non-reactive by EIA testing was found to be positive
       by either the OraSure HIV-1 WB or the licensed serum WB.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*DIAGNOSIS/PREVENTION &
       CONTROL  Antibodies, Viral/*ANALYSIS/BLOOD  Comparative Study  Human
       *HIV Seronegativity  HIV Seropositivity/BLOOD/*DIAGNOSIS
       HIV-1/*IMMUNOLOGY/ISOLATION & PURIF  Immunoenzyme Techniques  Mass
       Screening/METHODS  Mouth Mucosa/IMMUNOLOGY/*MICROBIOLOGY  Predictive
       Value of Tests  Specimen Handling/METHODS  MEETING ABSTRACT

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