Document 0830
 DOCN  M9480830
 TI    Genetic and serological analysis of Pneumocystis carinii genes encoding
       major surface glycoprotein (MSG).
 DT    9410
 AU    Sunkin SM; Stringer SL; Stringer JR; University of Cincinnati College of
       Medicine, Ohio.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:597 (abstract no. F-52). Unique
       Identifier : AIDSLINE ASM94/94313115
 AB    Pneumocystis carinii contains a large (90-120 kDa) surface glycoprotein
       that is recognized by antibodies in the serum of immune and infected
       hosts and seems to play a role in binding to host cells. Recent genetic
       analysis of MSG cDNAs has shown that MSG is likely to be a family of
       proteins. A fragment from the genome of rat-derived P. carinii was found
       to contain two MSG genes arranged as a direct repeat. The sequences from
       one gene (MSG B), the region between the two genes, and part of the
       second gene (MSG A) were determined. The open reading frames of MSG A
       and MSG B encode non-identical proteins, both of which are similar to
       that encoded by a previously published cDNA. The 5' and 3' untranslated
       regions of the MSG gene pair were highly conserved. Genetic analysis of
       MSG genes and cDNAs has assisted in defining presumptive variable and
       constant regions of the MSG proteins, which can then be utilized in
       examining the expression of this protein family. In another approach to
       define variable and constant regions, an expression library of
       rat-derived P. carinii was screened with monoclonal antibodies that
       recognize MSG from rat-derived and human-derived P. carinii. Some of
       these monoclonal antibodies appear to recognize an epitope that varies
       among MSG proteins, while two others may recognize epitopes that are
       conserved among these proteins.
 DE    Animal  Antibodies, Monoclonal  Antigenic Determinants/ANALYSIS
       Comparative Study  Fungal Proteins/ANALYSIS/BIOSYNTHESIS/*GENETICS  Gene
       Library  *Genes, Fungal  Human  Membrane
       Glycoproteins/ANALYSIS/BIOSYNTHESIS/*GENETICS  Open Reading Frames
       Pneumocystis carinii/*GENETICS/ISOLATION & PURIF/METABOLISM  Rats
       Repetitive Sequences, Nucleic Acid  Species Specificity  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).