Document 0835
 DOCN  M9480835
 TI    VIDAS vs ELISA in the serodiagnosis of HIV 1 + 2, rubella and
       toxoplasma.
 DT    9410
 AU    Araj GF; Bizri AR; Fattouh LG; Nassif RE; American Univ. Med. Ctr.,
       Beirut, Lebanon.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:555 (abstract no. C-365).
       Unique Identifier : AIDSLINE ASM94/94313110
 AB    Rapid, automated and self-contained instruments that minimize exposure
       to potentially infectious blood samples are attractive addition to the
       serodiagnostic laboratory. The performance of Vitek Immuno Diagnostic
       Assay Systems (VIDAS, biomerieux Vitek Inc., Hazelwood, MO) was tried in
       a developing country for the detection of antibodies to three infectious
       diseases. Among 87 selected sera which were tested for anti-HIV 1 + 2,
       20 and 13 (of the 20) were positive by ELISA (CLONATEC, Paris, France)
       and VIDAS, respectively. The Western blot (Lia Tek HIV 1 + 2, Organon
       Teknika, Boxtel, Holland) done on the 20 positive sera confirmed only
       the 13 which were also positive by VIDAS. Concordant results were
       obtained for 21 sera (12 positive) tested for anti-rubella IgG by ELISA
       (Human, Taunusstein, Germany) and VIDAS. Concerning toxoplasma ELISA
       (Human), IgM and IgG were positive for 5 of 19 and 27 of 27 sera,
       respectively, while VIDAS was positive for 1 of 19 and 23 of 27 sera,
       respectively. An arbitrator Toxo ELISA (Organon) for IgG and IgM
       confirmed the VIDAS results. The sera with discrepant Human Toxo ELISA
       IgG & IgM had O.D. readings close to the cut-off values. In addition to
       the above noted characteristics, the VIDAS is a reliable instrument to
       introduce in the clinical laboratories performing serodiagnosis of
       infectious diseases.
 DE    Acquired Immunodeficiency Syndrome/*DIAGNOSIS  Antibodies,
       Protozoan/BLOOD  Antibodies, Viral/BLOOD  Comparative Study
       Enzyme-Linked Immunosorbent Assay/*METHODS  Human  HIV Antibodies/BLOOD
       HIV-1/*ISOLATION & PURIF  HIV-2/*ISOLATION & PURIF  IgG/BLOOD  IgM/BLOOD
       Rubella/*DIAGNOSIS  Serodiagnosis/*METHODS  Toxoplasmosis/*DIAGNOSIS
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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