Document 0842
 DOCN  M9480842
 TI    Evaluation of a direct ELISA HIV-1/HIV-2 EIA using synthetic peptides:
       detect-HIV (version 2).
 DT    9410
 AU    Chalifour RJ; Ha NH; Barbeau S; Lacroix M; BioChem ImmunoSystems Inc.,
       Montreal, Que., Canada.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:554 (abstract no. C-362).
       Unique Identifier : AIDSLINE ASM94/94313103
 AB    A direct ELISA version of BioChem ImmunoSystems' synthetic peptide EIA
       was evaluated to establish if detecting all immunoglobulin classes
       improves the sensitivity of this test. HRP conjugates of 3 peptides from
       HIV-1 GP120, HIV-1 GP41 and HIV-2 GP36 were used to detect antibodies
       captured by these peptides on microtiter plates. Pending confirmatory
       testing, the specificity of this EIA was 99.5% (1974 fresh samples
       tested) while the sensitivity was 100% for HIV-1 (50/50) and 100% for
       HIV-2 (15/15). By testing serially diluted samples the sensitivity of
       the direct EIA was found to surpass that of the indirect EIA (version 1)
       with seroconversion samples and was of similar sensitivity with samples
       from later stages of HIV infection. The signal of both the direct and
       indirect EIAs was reduced by > 90% after IgG absorption by Protein G
       except with seroconversion samples where only a 30% signal reduction
       occurred with the direct EIA. This indicated that the improved
       sensitivity of the direct EIA with samples taken during seroconversion
       is because the direct EIA format detects IgG as well as other
       immunoglobulin classes.
 DE    Enzyme-Linked Immunosorbent Assay/*METHODS  Human  HIV Antibodies/BLOOD
       HIV Envelope Protein gp120/*ANALYSIS  HIV Envelope Protein
       gp41/*ANALYSIS  HIV Seropositivity/*DIAGNOSIS  HIV-1/*ISOLATION & PURIF
       HIV-2/*ISOLATION & PURIF  IgG/BLOOD  Peptides/CHEMICAL SYNTHESIS
       Sensitivity and Specificity  Viral Envelope Proteins/*ANALYSIS  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).