Document 0847
 DOCN  M9480847
 TI    Peripheral blood and milk-derived leukocytes harbor BIV provirus.
 DT    9410
 AU    Nash JW; Hanson LA; St. Cyr Coats K; Mississippi State University.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:486 (abstract no. T-22). Unique
       Identifier : AIDSLINE ASM94/94313098
 AB    Bovine immunodeficiency virus (BIV), a lentivirus, infects a large
       population of cattle in the southern United States. The mode of
       transmission of BIV is unknown, but its similarity to HIV suggests
       analogous mechanisms of transmission. The purpose of this study was to
       develop a PCR probe to detect BIV proviral sequences in cells which are
       likely vehicles for BIV transmission. DNA was prepared from peripheral
       blood (PBL) and milk-derived leukocytes and used as template for PCR.
       The proviral target for PCR amplification was a 234 bp fragment within a
       highly conserved region of the BIV pol gene. DNA fragments of
       approximately 234 bp were amplified from the DNA of both PBL and
       milk-derived leukocytes from BIV seropositive animals. Nucleotide
       sequencing of the amplified products confirmed their BIV specificity.
       Further study is necessary to determine the role of whole blood and milk
       in transmission of BIV in natural infections.
 DE    Animal  Base Sequence  Cattle  Cattle Diseases  Comparative Study
       Conserved Sequence  DNA, Viral/*ANALYSIS/BLOOD  Female  Genes, pol
       Immunodeficiency Virus, Bovine/GENETICS/*ISOLATION & PURIF  Lentivirus
       Infections/DIAGNOSIS/VETERINARY  Leukocytes/*MICROBIOLOGY
       Milk/*MICROBIOLOGY  Polymerase Chain Reaction/METHODS
       Proviruses/GENETICS/ISOLATION & PURIF  MEETING ABSTRACT

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