Document 0864
 DOCN  M9480864
 TI    Identification of three distinct HIV-1 subtypes in Brazil based on the
       protease gene.
 DT    9410
 AU    Janini LM; Luo CC; Peralta M; Schecther M; Kalish ML; Pieniazek N; de la
       Torre N; Schochetman G; Rayfield M; Pieniazek D; CDC, Atlanta, GA.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:482 (abstract no. T-1). Unique
       Identifier : AIDSLINE ASM94/94313081
 AB    OBJECTIVE: To study genotypic differences in the protease gene from
       HIV-1 infected persons in Brazil. METHODS: HIV-1 proviral DNA from 40
       specimens were surveyed for genetic similarity to reference strains
       using a PCR typing procedure based on the polymorphism of restriction
       sites in the p24 gag region and in the protease gene. Twenty four of
       these isolates were selected for protease gene sequencing. The sequence
       data were used to infer phylogenetic relationship between isolates using
       maximum likelihood and distance matrix methods. RESULTS: Three
       genetically distinct subtypes were identified on the basis of their
       protease gene sequences. Twenty one of the sequences were related to
       those of the North American/European env subtype (B). The remaining 3
       strains clustered into 2 distinct groups in the phylogenetic tree: one
       sequence clustered with the African strains ELI and Z2Z6 (env subtype
       D), whereas two other sequences clustered with strains in the env
       subtype F group. CONCLUSIONS: Analysis of protease gene demonstrated the
       existence of 3 distinct HIV-1 phylogenetic groups corresponding to env
       subtypes B, D and F in the Brazilian population in contrast to the two
       env subtypes (B and F) previously identified. Whether differences
       between the grouping of some Brazilian strains, based on protease or
       envelope gene regions, is a result of convergence in V3 region or a
       recombination event merits further studies.
 DE    Brazil  Comparative Study  DNA, Viral/*ISOLATION & PURIF  Genes, gag
       *Genes, Viral  Human  HIV Protease/*GENETICS
       HIV-1/*CLASSIFICATION/*GENETICS/ISOLATION & PURIF  Phylogeny  Polymerase
       Chain Reaction/METHODS  Polymorphism (Genetics)  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).