Document 0866
 DOCN  M9480866
 TI    The removal of human immunodeficiency virus (HIV) from fluids containing
       serum with a 0.04 micron nylon membrane filter.
 DT    9410
 AU    Oshima K; Comans T; Rowe T; Orloff S; Highsmith A; Ades E; Centers for
       Disease Control and Prevention, Atlanta, GA.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:424 (abstract no. Q-205).
       Unique Identifier : AIDSLINE ASM94/94313079
 AB    The risks associated with blood and blood products contaminated with
       human immunodeficiency virus (HIV) has been an ongoing concern in the
       health care industry. We tested the ability of a 0.04 micron nylon
       membrane filter to remove human immunodeficiency virus (HIV) from fluid
       containing serum and characterized the effect of filtration on the
       concentration of serum proteins. The filters were tested with HIV
       suspended in Dulbecco's modified minimum essential medium (DMEM)
       containing 10% fetal bovine serum. Endpoint titrations of infectious
       virus (ID50) were performed on lymphocyte cultures. Virus concentrations
       were determined using an enzyme-linked immunoabsorbent assay (ELISA) of
       the HIV-1 P24 core antigen. In three replicate challenges with 47-mm
       membrane disks, no virus was detected in the filtrate. The titer
       reduction was estimated to be greater than 8.5 x 10(2). The
       concentration of IgM (900,000 Da), IgG (160,000 Da), and albumin (67,000
       Da) in pooled human serum was determined by ELISA before and after 0.04
       micron nylon membrane filtration. High recovery efficiency of these
       proteins were observed. No relationship between the molecular weight of
       the protein and recovery was detected. The results suggest that the pore
       size distribution of the 0.04 micron membrane filter is sufficient to
       allow biologically active serum proteins through during filtration.
 DE    Blood/*MICROBIOLOGY  Blood Proteins/ANALYSIS  Enzyme-Linked
       Immunosorbent Assay  Human  HIV/*ISOLATION & PURIF  HIV Core Protein
       p24/ANALYSIS  HIV-1/*ISOLATION & PURIF  IgM/BLOOD  Nylons  Risk Factors
       Serum Albumin/ANALYSIS  Ultrafiltration/*METHODS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).