Document 0869
 DOCN  M9480869
 TI    T cell reactivity against antigen 85 in HIV-infection.
 DT    9410
 AU    Huygen K; Gerard M; Farber CM; Drowart A; van Vooren JP; Clumeck N;
       Pasteur Instituut van Brabant, Brussels, Belgium.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:190 (abstract no. U-100).
       Unique Identifier : AIDSLINE ASM94/94313076
 AB    Antigen 85 (30-32 kD) is the major secreted protein antigen from M.
       tuberculosis/bovis BCG culture filtrate. We have previously shown that
       32 kD component (Ag85A) of this antigen demonstrates powerful T cell
       stimulatory properties resulting in T cell proliferation and IFN-gamma
       secretion by PBMC from all healthy tuberculin-positive volunteers and by
       only a minority of tuberculosis patients, suggesting a protective role
       of T cell immunity to Ag85 in tuberculous infection (1). Endogenous
       reactivation of latent M. tuberculosis, exogenous infection with the
       Koch bacillus and disease caused by mycobacteria other than M.
       tuberculosis (MOTT) form a serious health problem in HIV-infection.
       Predictive markers of increased risk for developing disease, other than
       CD4 counts, are not defined for the moment. The purpose of this study is
       to analyze T cell reactivity against Ag85 in tuberculin-negative (ID-)
       and tuberculin-positive (ID+) HIV-seropositive subjects (CDC stages II
       and III) and in AIDS patients (CDC IV C1 and IV C2). TABULAR DATA, SEE
       ABSTRACT VOLUME. *: number of individuals that demonstrated a positive
       in vitro T cell response to PPD or Ag85. In stage CDC IV C1, six
       patients suffered from MOTT and all were unresponsive to PPD and Ag85.
       One patient with extrapulmonary TB was reactive to PPD but not to Ag85.
       These results suggest that decreased T cell reactivity against Ag85
       occurs early during HIV infection, even before decreases in PPD
       responsiveness can be measured. A follow-up study on the HIV+/ID+ cohort
       will indicate whether T cell reactivity against the antigen can be used
       as a predictive marker of mycobacterial complications.
 DE    Acquired Immunodeficiency Syndrome/*IMMUNOLOGY  Antigens,
       Bacterial/*IMMUNOLOGY  AIDS-Related Opportunistic
       Infections/EPIDEMIOLOGY/IMMUNOLOGY  Comparative Study  Human  HIV
       Seropositivity/*IMMUNOLOGY  *Lymphocyte Transformation  Mycobacterium
       bovis/*IMMUNOLOGY  Predictive Value of Tests  Risk Factors
       T-Lymphocytes/*IMMUNOLOGY  Tuberculin Test
       Tuberculosis/*EPIDEMIOLOGY/MICROBIOLOGY  T4 Lymphocytes/*IMMUNOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).